| 货号 | MAB9311-SP |
| 别名 | APP beta-secretase; ASP2; Aspartyl protease 2; BACEAsp 2; beta-secretase 1 precursor variant 1; beta-secretase 1; beta-site amyloid beta A4 precursor protein-cleaving enzyme; Beta-site amyloid precursor protein cleaving enzyme 1; Beta-site APP cleaving enzyme 1; beta-site APP-cleaving enzyme 1; beta-site APP-cleaving enzyme; EC 3.4.23; EC 3.4.23.46; FLJ90568; HSPC104; KIAA1149; memapsin-2; Membrane-associated aspartic protease 2; transmembrane aspartic proteinase Asp2 | 全称 | beta-site Ayloid Precursor Protein Cleaving Enzyme 1 |
| 反应种属 | Human/Mouse |
| 应用 | Immunoprecipitation,Neutralization |
| 目标/特异性 | Detects human and mouse BACE‑1 Ectodomain in direct ELISAs. In direct ELISAs, no cross‑reactivity with recombinant human (rh) BACE-2, recombinant mouse BACE-2, rhADAM8, rhADAM9, rhADAM10, rhADAM15, or rhTACE is observed. |
| 使用方法 | Immunoprecipitation: 25 µg/mL Neutralization: Measured by its ability to neutralize Recombinant Human BACE‑1 (10 µg/mL, Catalog # 931-AS) or Recombinant Mouse BACE‑1 (10 µg/mL, Catalog # 2976-AS) cleavage of the fluorogenic peptide substrate Mca-SEVNLDAEFRK(Dnp)RR-NH2 (10 µM, Catalog # ES004). The Neutralization Dose (ND50) is typically 10 µg/mL. |
| 来源 | Monoclonal Mouse IgG1 Clone # 137626 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 23621 (Human); 23821 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. A reversible form of lysine acetylation in the ER and Golgi lumen controls the molecular stabilization of BACE1. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human BACE-1 Thr22-Tyr460 Accession # P56817 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | BACE-1(beta‑site APP cleaving enzyme-1) is an aspartic protease and an integral membrane protein (1, 2). It is the major beta secretase, and together with the gamma secretase, is responsible for generating the amyloid beta peptide (A beta ) from the amyloid precursor protein (APP) (3, 4). Because A beta is a major component of amyloid plaques, BACE-1 has been implicated in the onset and/or progression of Alzheimers disease. High levels of BACE-1 activity are sufficient to elicit neurodegeneration and neurological decline in vivo, indicating that inhibiting BACE-1 may block not only A beta -dependent but also A beta -independent pathogenic mechanisms (5). In addition to APP, BACE-1 also cleaves APP-like proteins 1 and 2, the cell adhesion protein P-selectin glycoprotein ligand-1 and beta -galactoside alpha 2,6-sialyltransferase, implying that BACE-1 may have additional functions involving the ectodomain shedding of membrane proteins (6 - 8). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Neutralization of BACE‑1 Activity by Human/Mouse BACE‑1 Antibody. The cleavage of Mca-SEVNLDAEFRK(Dnp)RR-NH2 (10 µM, Catalog # ES004) by Recombinant Human BACE‑1 (10 µg/mL, Catalog # 931-AS) or Recombinant Mouse BACE‑1 (10 µg/mL, Catalog # 2976-AS) is measured after preincubation with increasing concentrations of Mouse Anti-Human/Mouse BACE‑1 Ectodomain Monoclonal Antibody (Catalog # MAB9311). The ND50 is typically 10 µg/mL.a |
