| 货号 | MAB165-SP |
| 别名 | B7H2B7 homolog 2; B7-H2B7-related protein 1; B7RP-1; B7RP1B7-like protein Gl50; B7RP-1LICOS; CD275 antigen; CD275; GL50; GL50transmembrane protein B7-H2 ICOS ligand; ICOSL; ICOS-L; ICOSLG; inducible T-cell co-stimulator ligand; KIAA0653ICOS ligand | 全称 | B7 Homolog 2 |
| 反应种属 | Human |
| 应用 | Flow Cytometry,CyTOF-ready,Neutralization |
| 目标/特异性 | Detects human B7-H2 in direct ELISAs. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) B7-1, rhB7-2, rhB7-H1, rhB7-H3, or recombinant mouse B7-H2 is observed. |
| 使用方法 | Flow Cytometry: 2.5 µg/106cells Neutralization: Measured by its ability to neutralize B7‑H2-induced proliferation in PHA-activated human T cells. Wang, S. et al. (2000) Blood 96:2808. The Neutralization Dose (ND50) is typically 1-4 µg/mL in the presence of 3 µg/mL Recombinant Human B7‑H2 Fc Chimera and 20 ng/mL Human CD3 epsilon Monoclonal Antibody. |
| 来源 | Monoclonal Mouse IgG2B Clone # 136726 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 23308 (Human); 50723 (Mouse); 102132485 (Cynomolgus Monkey) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. ICOS-LICOS interaction is critically involved in TGN1412-mediated T-cell activation. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human B7-H2 Asp19-Ser258 Accession # O75144 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Human B7-H2, also called B7RP-1, B7h, LICOS, and GL50, is a 60 kDa member of the B7 family of immune costimulatory proteins, which includes B7-1, B7-2, B7-H1 (PD-L1), PD-L2, and B7-H3. B7 proteins are members of the immunoglobulin (Ig) superfamily, the extracellular domains contain 2 Ig-like domains and all members have short cytoplasmic domains. Family members share about 20‑25% amino acid identity. Within the extracellular domain, human B7-H2 shares 49% and 54% amino acid sequence identity with mouse and rat B7-H2, respectively. B7-H2 has been identified as the ligand for ICOS, a member of the CD28 family of costimulatory receptors. Human B7-H2 is a 309 amino acid (aa) protein with a putative 18 aa signal peptide, a 239 aa extracellular domain, an 18 aa transmembrane region, and a 33 aa cytoplasmic domain. Human B7-H2 is expressed constitutively on resting B cells, dendritic cells, and at low levels on monocytes. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and Thdifferentiation. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Cell Proliferation Induced by B7‑H2 and Neutralization by Human B7‑H2 Antibody. In the presence of Human CD3 epsilon Monoclonal Antibody (20 ng/mL, Catalog # MAB100), Recombinant Human B7‑H2 Fc Chimera (Catalog # 165-B7) stimulates proliferation in PHA-activated human T cells in a dose-dependent manner (orange line). Under these conditions, proliferation elicited by Recombinant Human B7‑H2 Fc Chimera (3 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human B7‑H2 Monoclonal Antibody (Catalog # MAB165). The ND50 is typically 1-4 µg/mL. | |
Detection of B7‑H2 in U937 Human Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human B7‑H2 Monoclonal Antibody (Catalog # MAB165, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab)2 Secondary Antibody (Catalog # F0102B). |
