Human/Mouse/Rat Activin A (bA Subunit) MAb (Clone 69403) (25 UG)【科瑞奥博】

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Human/Mouse/Rat Activin A (bA Subunit) MAb (Clone 69403) (25 UG)

货号： MAB3381-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB3381-SP
别名	INHBA
反应种属	Human/Mouse/Rat
应用	ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),Neutralization
目标/特异性	Detects recombinant human Activin A in ELISAs. Also recognizes Activin A precursor. The amino acid sequences of mature beta _A subunits from human, mouse and rat are identical. Therefore, this antibody will also detect the mature beta _A subunit from mouse and rat.
使用方法	ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.5-2.0 µg/mL Neutralization: Measured by its ability to neutralize Activin A-induced hemoglobin expression in the K562 human chronic myelogenous leukemia cell line. The Neutralization Dose (ND₅₀) is typically 0.02-0.06 µg/mL in the presence of 7.5 ng/mL Recombinant Human/Mouse/Rat Activin A.
来源	Monoclonal Mouse IgG₁ Clone # 69403
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3624 (Human); 16323 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Activin A programs the differentiation of human TFH cells Nat Immunol, 2016;17(8):976-84. Species: Human Sample Type: Whole Cells Application: Bioassay Low miR-143/miR-145 Cluster Levels Induce Activin A Overexpression in Oral Squamous Cell Carcinomas, Which Contributes to Poor Prognosis. Authors: Bufalino A, Cervigne N, de Oliveira C, Fonseca F, Rodrigues P, Macedo C, Sobral L, Miguel M, Lopes M, Paes Leme A, Lambert D, Salo T, Kowalski L, Graner E, Coletta R PLoS ONE, 2015;10(8):e0136599. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development Adipose Stromal Cell Contact with Endothelial Cells Results in Loss of Complementary Vasculogenic Activity Mediated by Induction of Activin A. Authors: Merfeld-Clauss S, Lupov I, Lu H, March K, Traktuev D Stem Cells, 2015;33(10):3039-51. Species: Human Sample Type: Cell Culture Supernates Application: Neut Activin A skews macrophage polarization by promoting a proinflammatory phenotype and inhibiting the acquisition of anti-inflammatory macrophage markers. Authors: Sierra-Filardi E, Puig-Kroger A, Blanco FJ, Nieto C, Bragado R, Palomero MI, Bernabeu C, Vega MA, Corbi AL Blood, 2011;117(19):5092-101. Species: Human Sample Type: Whole Cells Application: Neut The neuroprotective effect of Activin A and B: implication for neurodegenerative diseases. Authors: Kupershmidt L, Amit T, Bar-Am O, Youdim MB, Blumenfeld Z J. Neurochem., 2007;103(3):962-71. Species: Human Sample Type: Cell Lysates Application: WB Activin A is an anticatabolic autocrine cytokine in articular cartilage whose production is controlled by fibroblast growth factor 2 and NF-kappaB. Authors: Alexander S, Watt F, Sawaji Y, Hermansson M, Saklatvala J Arthritis Rheum., 2007;56(11):3715-25. Species: Human Sample Type: Whole Cells Application: Neut Requirement of Smad3 for mast cell growth. Authors: Funaba M, Nakaya K, Ikeda T, Murakami M, Tsuchida K, Sugino H Cell. Immunol., 2006;240(1):47-52. Species: Mouse Sample Type: Cell Lysates Application: WB Fibroblast growth factor 2 modulates transforming growth factor beta signaling in mouse embryonic fibroblasts and human ESCs (hESCs) to support hESC self-renewal. Authors: Greber B, Lehrach H, Adjaye J Stem Cells, 2006;25(2):455-64. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Development N-cadherin is regulated by activin A and associated with tumor aggressiveness in esophageal carcinoma. Authors: Utsunomiya T, Sonoda H, Mimori K Clin. Cancer Res., 2004;10(17):5702-7. Species: Human Sample Type: Tissue Homogenates Application: WB
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Chinese hamster ovary cell line CHO-derived recombinant human Activin A
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human, Mouse, Rat
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Activin and Inhibin are members of the TGF-beta superfamily of cytokines and are involved in a wide range of biological processes including tissue morphogenesis and repair, fibrosis, inflammation, neural development, hematopoiesis, reproductive system function, and carcinogenesis (1‑7). Activin and Inhibin are produced as precursor proteins. Their amino terminal propeptides are proteolytically cleaved and facilitate formation of disulfide-linked dimers of the bioactive proteins (8, 9). Activins are nonglycosylated homodimers or heterodimers of various beta subunits ( beta A, beta B, beta C, and beta E in mammals), while Inhibins are heterodimers of a unique alpha subunit and one of the beta subunits. Activin A is a widely expressed homodimer of two beta A chains. The beta A subunit can also heterodimerize with a beta B or beta C subunit to form Activin AB and Activin AC, respectively (10). The 14 kDa mature human beta A chain shares 100% amino acid sequence identity with bovine, feline, mouse, porcine, and rat beta A. Activin A exerts its biological activities by binding to the type 2 serine/threonine kinase Activin RIIA which then noncovalently associates with the type 1 serine/threonine kinase Activin RIB/ALK-4 (7, 11). Signaling through this receptor complex leads to Smad activation and regulation of activin-responsive gene transcription (7, 11). The bioactivity of Activin A is regulated by a variety of mechanisms (11). BAMBI, Betaglycan, and Cripto are cell-associated molecules that function as decoy receptors or limit the ability of Activin A to induce receptor complex assembly (12‑14). The intracellular formation of Activin A can be prevented by the incorporation of the beta A subunit into Activin AC or Inhibin A (3, 10). And the bioavailability of Activin A is restricted by its incorporation into inactive complexes with alpha 2-Macroglobulin, Follistatin, and FLRG (15, 16).
运输条件	Blue Ice
存放说明	4℃
参考文献	Kumanov, P. et al. (2005) Reprod. Biomed. Online 10:786. Maeshima, A. et al. (2008) Endocr. J. 55:1. Rodgarkia-Dara, C. et al. (2006) Mutat. Res. 613:123. Werner, S. and C. Alzheimer (2006) Cytokine Growth Factor Rev. 17:157. Xu, P. and A.K. Hall (2006) Dev. Biol. 299:303. Shav-Tal, Y. and D. Zipori (2002) Stem Cells 20:493. Chen, Y.G. et al. (2006) Exp. Biol. Med. 231:534. Gray, A.M. and A.J. Mason (1990) Science 247:1328. Mason, A.J. et al. (1996) Mol. Endocrinol. 10:1055. Thompson, T.B. et al. (2004) Mol. Cell. Endocrinol. 225:9. Harrison, C.A. et al. (2005) Trends Endocrinol. Metab. 16:73. Onichtchouk, D. et al. (1999) Nature 401:480. Gray, P.C. et al. (2002) Mol. Cell. Endocrinol. 188:254. Kelber, J.A. et al. (2008) J. Biol. Chem. 283:4490. Phillips, D.J. et al. (1997) J. Endocrinol. 155:65. Schneyer, A. et al. (2003) Endocrinology 144:1671.

参考图片

Hemoglobin Expression Induced by Activin A and Neutralization by Human/
Mouse/Rat Activin A Antibody.

Recombinant Human/Mouse/
Rat Activin A (Catalog # 338-AC) increases hemoglobin expression in the K562 human chronic myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the psuedoperoxidase assay. Hemoglobin Expression elicited by Recombinant Human/Mouse/Rat Activin A (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human/Mouse/Rat Activin A beta A subunit Monoclonal Antibody (Catalog # MAB3381). The ND₅₀ is typically 0.02-0.06 µg/mL.