Human BMP-2/4 MAb (Clone 100230) (25 UG)【科瑞奥博】

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Human BMP-2/4 MAb (Clone 100230) (25 UG)

货号： MAB3552-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB3552-SP
别名	BDA2; BMP-2; BMP2A; BMP-2A; BMP2ABone morphogenetic protein 2A; bone morphogenetic protein 2
全称	Bone Morphogenetic Protein 2/4
反应种属	Human
应用	Western Blot,Neutralization
目标/特异性	Detects human BMP-2 and human BMP-4 in ELISAs and Western blots. Shows 100% cross-reactivity with recombinant zebra fish (rzf) BMP-2 and rhBMP-4, 20%-40% cross-reactivity with rhBMP-3, -3b, -6, and no cross-reactivity with rhBMP-5, -10, or -15.
使用方法	Western Blot: 1 µg/mL Neutralization: Measured by its ability to neutralize BMP‑2-induced alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The Neutralization Dose (ND₅₀) is typically 5-15 µg/mL in the presence of 1 µg/mL Recombinant Human BMP‑2 and 2 µg/mL heparin. This antibody also neutralizes Human BMP-4 induced activity in the ATDC5 mouse chondrogenic cell line. The ND₅₀ is approximately 3 μg/mL in the presence of 75 ng/mL of Recombinant Human BMP-4.
来源	Monoclonal Mouse IgG₁ Clone # 100230
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	650 (Human); 12156 (Mouse); 29373 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. The BMP Pathway Participates in Human Naive CD4+ T Cell Activation and Homeostasis. Authors: Martinez V, Sacedon R, Hidalgo L, Valencia J, Fernandez-Sevilla L, Hernandez-Lopez C, Vicente A, Varas A PLoS ONE, 2015;10(6):e0131453. Species: Human Sample Type: Whole Cells Application: Bioassay Essential role of BMPs in FGF-induced secondary lens fiber differentiation. Authors: Boswell BA, Overbeek PA, Musil LS Dev. Biol., 2008;324(2):202-12. Species: Chicken Sample Type: Whole Cells Application: Neut Regulation of bone morphogenetic protein signalling in human pulmonary vascular development. Authors: Southwood M, Jeffery TK, Yang X, Upton PD, Hall SM, Atkinson C, Haworth SG, Stewart S, Reynolds PN, Long L, Trembath RC, Morrell NW J. Pathol., 2008;214(1):85-95. Species: Human Sample Type: Whole Tissue Application: IHC Nephroblastoma overexpressed (Nov) inhibits osteoblastogenesis and causes osteopenia. Authors: Rydziel S, Stadmeyer L, Zanotti S, Durant D, Smerdel-Ramoya A, Canalis E J. Biol. Chem., 2007;282(27):19762-72. Species: Mouse Sample Type: GST and GST-Nov Fusion proteins Application: WB Iron transferrin regulates hepcidin synthesis in primary hepatocyte culture through hemojuvelin and BMP2/4. Authors: Lin L, Valore EV, Nemeth E, Goodnough JB, Gabayan V, Ganz T Blood, 2007;110(6):2182-9. Species: Mouse Sample Type: Whole Cells Application: Neut Combinatorial gene therapy for bone regeneration: cooperative interactions between adenovirus vectors expressing bone morphogenetic proteins 2, 4, and 7. Authors: Zhao M, Zhao Z, Koh JT, Jin T, Franceschi RT J. Cell. Biochem., 2005;95(1):1-16. Species: Mouse Sample Type: Cell Culture Supernates Application: IP
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Chinese hamster ovary cell line CHO-derived recombinant human BMP-2 Gln283-Arg396 Accession # P12643
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Bone Morphogenetic Protein 2 (BMP-2) and BMP-4 are two of at least 15 structurally and functionally related BMPs, which are members of the transforming growth factor beta (TGF-beta ) superfamily, BMPs were originally identified as protein regulators of cartilage and bone formation. However, they have since been shown to be involved in embryogenesis and morphogenesis of various tissues and organs.
运输条件	Blue Ice
存放说明	4℃
参考文献	BMP Family BMPs BMPs & Activins Influence FSH Synthesis BMPs (Bone Morphogenetic Proteins) Cytokines in the Nervous System Early Mesodermal Lineage Markers Mini-review: Bone Morphogenetic Proteins (BMPs) TGF-beta Superfamily

参考图片

Alkaline Phosphatase Production Induced by BMP‑2 and Neutralization by Human BMP-2/BMP-4 Antibody.

Recombinant Human BMP‑2 (Catalog # 355-BM) induces alkaline phosphatase production in the the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline Phosphatase Production elicited by Recombinant Human BMP‑2 (1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human BMP-2/BMP-4 Monoclonal Antibody (Catalog # MAB3552). The ND₅₀ is typically 5-15 µg/mL in the presence of heparin (2 µg/mL).