Human EGF MAb (Clone 10825) (25 UG)【科瑞奥博】

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Human EGF MAb (Clone 10825) (25 UG)

货号： MAB236-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB236-SP
别名	beta-urogastrone; epidermal growth factor (beta-urogastrone); epidermal growth factor; HOMG4; pro-epidermal growth factor; URG; Urogastrone
全称	Epidermal Growth Factor
反应种属	Human
应用	Western Blot,Immunohistochemistry,Neutralization
目标/特异性	Detects human EGF in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) HB‑EGF or rhTGF‑ alpha is observed.
使用方法	Western Blot: 1 µg/mL Immunohistochemistry: 8-25 µg/mL Neutralization: Measured by its ability to neutralize EGF-induced proliferation in the Balb/3T3 mouse embryonic fibroblast cell line. The Neutralization Dose (ND₅₀) is typically 0.05-0.1 µg/mL in the presence of 2 ng/mL Recombinant Human EGF.
来源	Monoclonal Mouse IgG₁ Clone # 10825
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	1950 (Human); 13645 (Mouse); 25313 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Anti-inflammatory activity of nanocrystalline silver-derived solutions in porcine contact dermatitis. Authors: Nadworny PL, Wang J, Tredget EE J Inflamm (Lond), 2010;7(0):13. Species: Porcine Sample Type: Whole Tissue Application: IHC Paraffin-embedded S100A11, an dual mediator for growth regulation of human keratinocytes. Authors: Sakaguchi M, Sonegawa H, Murata H, Kitazoe M, Futami J, Kataoka K, Yamada H, Huh NH Mol. Biol. Cell, 2007;19(1):78-85. Species: Human Sample Type: Cell Lysates Application: WB Transforming growth factor-alpha: a major human serum factor that promotes human keratinocyte migration. Authors: Li Y, Fan J, Chen M, Li W, Woodley DT J. Invest. Dermatol., 2006;126(9):2096-105. Species: Human Sample Type: Serum Application: Neut Salivary leptin induces increased expression of growth factors in oral keratinocytes. Authors: Groschl M, Topf HG, Kratzsch J, Dotsch J, Rascher W, Rauh M J. Mol. Endocrinol., 2005;34(2):353-66. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant human EGF
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	EGF is the prototypic member of a family of growth factors that arecharacterized by the presence of EGF like domains and activate membersof the EGF receptor family. Proteolytic cleavage of a membrane-boundprecursor releases mature soluble EGF which interacts with the EGF R topromote proliferation and differentiation of mesenchymal and epithelialcells.
运输条件	Blue Ice
存放说明	4℃
参考文献	Cancer Biomarkers Cytokines and Receptors in Angiogenesis EGF Family EGF Ligands EMT Induction gamma delta T Cells ICC / IHC Images: EGF Inflammatory Mediators TrkB Integrates Neural Precursor Cell Migration & Differentiation in the Developing Brain

参考图片

EGF in Human Skin. EGF was detected in immersion fixed paraffin-embedded sections of human skin using 25 µg/mL Mouse Anti-Human EGF Monoclonal Antibody (Catalog # MAB236) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of keratinocytes in the stratum granulosum in epidermis. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Cell Proliferation Induced by EGF and Neutralization by Human EGF Antibody. Recombinant Human EGF (Catalog # 236-EG) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human EGF (2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human EGF Monoclonal Antibody (Catalog # MAB236). The ND₅₀ is typically
0.05‑0.1 µg/mL.