| 货号 | MAB6961-SP |
| 别名 | CCI | 全称 | CC Chemokine Inhibitor |
| 应用 | Western Blot,Neutralization |
| 目标/特异性 | Detects viral CCI in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Neutralization: Measured by its ability to neutralize CCI inhibition of CCL2/JE/MCP‑1-dependent chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR2A. 6 μg/mL of this antibody will neutralize > 60 % of 0.4 μg/mL Recombinant Viral CCI Fc Chimera activity in the presence of 20.0 ng/mL Recombinant Mouse CCL2/JE/MCP‑1.
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| 来源 | Monoclonal Mouse IgG1 Clone # 112803 |
| 产品组分 |
| 供应商 | R&D Systems |
| 纯化方式 | Protein A or G purified from ascites |
| 免疫原 | S. frugiperda insect ovarian cell line Sf 21-derived recombinant viral CCI Met17-Val258 Accession # P19063 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Viral |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | The family of T1/35 kDa proteins are secreted, soluble proteins encoded by the 35K virulence gene of many poxviruses. These proteins have been shown to bind CC-chemokines with high affinity and are now termed viral chemokine inhibitor (vCCI). Viral CCI from various poxviruses share multiple stretches of identical sequence motif and eight conserved cysteine residues. The vaccinia virus (strain Lister) vCCI cDNA encodes a 258 amino acid (aa) residue protein with a putative 17 amino acid residue signal peptide. Vaccinia virus (strain Lister) vCCI shows greater than 90% aa sequence identity with vCCI from other orthopoxviruses and approximately 40% aa sequence identity with the leporipoxvirus T-1 proteins. Recombinant vCCI has been shown to be a potent general inhibitor of CC-chemokine activity in vitro and blocks binding of CC chemokines to cell surface chemokine receptors. In in vivo studies using a virus mutant in which the gene encoding the CCI has been deleted, leukocyte infiltration into the virus-infected areas is increased, suggesting that CCI can modulate the influx of inflammatory cells into virus-infected tissues. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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CCI Inhibition of CCL2/JE-dependent Chemotaxis and Neutralization by Viral CCI Antibody. Recombinant Viral CCI Fc Chimera (Catalog # 696-CC) inhibits Recombinant Mouse CCL2/JE (Catalog # 479-JE) induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Inhibition of Recombinant Mouse CCL2/JE (20 ng/mL) activity elicited by Recombinant Viral CCI Fc Chimera (0.4 µg/mL) is neutralized (green line) by increasing concentrations of Viral CCI Monoclonal Antibody (Catalog # MAB6961). 6 μg/mL of this antibody will neutralize 60 % Recombinant Viral CCI Fc Chimera activity. |
