| 货号 | MAB7551-SP |
| 别名 | DAN domain family member 1; DAND1; MGC8972; NBL1; neuroblastoma suppressor of tumorigenicity 1; neuroblastoma, suppression of tumorigenicity 1; Protein N03; suppression of tumorigenicity 1; Zinc finger protein DAN | 全称 | Differential Screening-selected Gene Aberrant in Neuroblastoma |
| 反应种属 | Mouse |
| 应用 | Western Blot,Blockade of Receptor-ligand Interaction |
| 目标/特异性 | Detects mouse DAN in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) DAN, recombinant chicken Caronte, rhCerberus, or recombinant mouse Gremlin is observed. |
| 使用方法 | Western Blot: 1 µg/mL Blockade of Receptor-ligand Interaction: In a functional ELISA, 5-20 µg/mL of this antibody will block 50% of the binding of 100 ng/mL of Recombinant Human BMP-4 (Catalog # 314-BP) to immobilized Recombinant Mouse DAN (Catalog # 755-DA) coated at 2 µg/mL (100 µL/well). At 200 μg/mL, this antibody will block >90% of the binding. |
| 来源 | Monoclonal Rat IgG2A Clone # 123202 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4681 (Human); 17965 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse DAN Ala17-Asp178 Accession # Q61477.2 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | DAN(differential screening-selected gene aberrative in neuroblastoma) was initially identified as a gene whose expression is downregulated in src-transformed rat fibroblasts. DAN has now been shown to be a prototypical member of the DAN family of secreted glycoproteins that are putative BMP antagonists. DAN family members share a cysteine-rich domain that is structurally related to the cysteine-knot motif found in TGF-beta superfamily ligands. There are at least five mouse DAN family members, including DAN, Gremlin/DRM, Cer1 (Cerberus-related), Dante and PRDC (protein related to DAN and cerberus). Additional DAN family members include Xenopus Cerberus, chick Caronte, and C. elegans CeCan 1. Murine DAN binds BMP-2 in immunoprecipitation experiments and acts as a BMP antagonist in Xenopus animal cap explants. The DAN family of proteins are thought to act as antagonists by binding BMP ligands and preventing their interactions with signaling receptor complexes. Recombinant mouse DAN preparations from R&D Systems have been shown to bind BMP-4 in a functional ELISA. It is likely the various DAN family members and other TGF-beta BMP antagonists including Noggin, Chordin, Follistatin, and TSG can selectively antagonize the activities of different subsets of TGF-beta superfamily ligands. These antagonists represent one of the many elaborate regulatory mechanisms that have evolved to control the bioactivities of the TGF-beta superfamily ligands. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
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