Mouse CX3CL1/Fractalkine MAb (Clone 126315) (25 UG)【科瑞奥博】

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Mouse CX3CL1/Fractalkine MAb (Clone 126315) (25 UG)

货号： MAB571-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB571-SP
别名	ABCD-3; C3Xkine; chemokine (C-X3-C motif) ligand 1; CX3C membrane-anchored chemokine; CXC3; CXC3C; FKN; Fractalkine; Neurotactin; neurotactin); NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine; NTTSmall-inducible cytokine D1; SCYD1C-X3-C motif chemokine 1; small inducible cytokine subfamily D (Cys-X3-Cys), member-1
反应种属	Mouse
应用	Western Blot,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard ,Neutralization
目标/特异性	Detects mouse CX3CL1/Fractalkine in Western blots. In direct ELISAs, this antibody shows 100% cross-reactivity with the chemokine domain of recombinant human (rh) CX3CL1, 13% cross-reactivity with rhCX3CL1, and 50% cross-reactivity with recombinant rat CX3CL1.
使用方法	Western Blot: 1 µg/mL ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize CX3CL1/Fractalkine-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CX3CR1. The Neutralization Dose (ND₅₀) is typically 0.75-3.5 µg/mL in the presence of 30 ng/mL Recombinant Mouse CX3CL1/Fractalkine aa 25‑105.
来源	Monoclonal Rat IgG_2A Clone # 126315
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	6376 (Human); 20312 (Mouse); 89808 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Extracellular adenosine signaling induces CX3CL1 expression in the brain to promote experimental autoimmune encephalomyelitis. J Neuroinflammation, 2012;9(0):193. Species: Mouse Sample Type: In Vivo Application: In vivo Gene therapy with CX3CL1/Fractalkine induces antitumor immunity to regress effectively mouse hepatocellular carcinoma. Authors: Tang L, Hu HD, Hu P, Lan YH, Peng ML, Chen M, Ren H Gene Ther., 2007;14(16):1226-34. Species: Mouse Sample Type: Whole Cells Application: ICC
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Mouse myeloma cell line NS0-derived recombinant mouse CX3CL1/Fractalkine, S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse CX3CL1/Fractalkine, and E. coli-derived recombinant mouse CX3CL1/Fractalkine
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	CX3CL1, also known as Fractalkine, is a type I membrane protein in which a chemokine domain possessing a unique C-X3-C cysteine motif is tethered on a long mucin-like stalk. It can also be released as a soluble molecule upon proteolysis at a membrane proximal site.
运输条件	Blue Ice
存放说明	4℃
参考文献	Cancer Stem Cell Markers Chemokines and Receptors Chemokines and Receptors in Allergy and Asthma Chemokines and Receptors in Angiogenesis CX3C Chemokines and Receptors Fractalkine in Glomerulonephritis Fractalkine Signaling Modulates Neurotoxicity in vivo Glomerulonephritis: Beyond Inflammation ICC / IHC Images: CX3CL1/Fractalkine Macrophage Activation Markers Neuroinflammation Renal Markers Special Glycoproteins

参考图片

Chemotaxis Induced by CX3CL1/Fractalkine and Neutralization by Mouse CX3CL1/Fractalkine Antibody.

Recombinant Mouse CX3CL1/Fractalkine (Catalog # 571-MF) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CX3CR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CX3CL1/Fractalkine (30 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CX3CL1/Fractalkine Chemokine Domain Monoclonal Antibody (Catalog # MAB571). The ND₅₀ is typically 0.75‑3.5 µg/mL.