Human IL-18 R beta/IL-1 R7 MAb (Clone 132016) (25 UG)【科瑞奥博】

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Human IL-18 R beta/IL-1 R7 MAb (Clone 132016) (25 UG)

货号： MAB1181-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB1181-SP
别名	Accessory protein-like; AcPL; CD218 antigen-like family member B; CD218b antigen; CD218b; CDw218b; IL-1 R7; IL-18 receptor accessory protein; IL-18 receptor beta; IL-18RAcP; IL18RAP; IL18RB; IL-18Rbeta; IL-18R-beta; IL-1R accessory protein-like; IL1R7; IL-1R7; IL-1R-7; IL-1RAcPL; interleukin 18 receptor accessory protein; Interleukin-1 receptor 7; interleukin-18 receptor accessory protein; Interleukin-18 receptor accessory protein-like; Interleukin-18 receptor beta; MGC120589; MGC120590
全称	Interleukin 18 Receptor beta
反应种属	Human
应用	Western Blot,Neutralization
目标/特异性	Detects human IL‑18 R beta /IL‑1 R7 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant mouse IL-18 R beta, recombinant human (rh) IL-18 R, rhIL-1 RI, rhIL-1 RII, rhIL-1 RAcP, and rhIL-1 Rrp2 is observed.
使用方法	Western Blot: 1 µg/mL Neutralization: Measured by its ability to neutralize IL-18/IL-1F4-induced IFN-gamma secretion in the KG-1 human acute myelogenous leukemia cell line. Novick, D. et al. (1999) Immunity 10:127. The Neutralization Dose (ND₅₀) is typically 0.3-1.0 μg/mL in the presence of 40 ng/mL Recombinant Human IL-18/IL-1F4 and 20 ng/mL Recombinant Human TNF-alpha.
来源	Monoclonal Mouse IgG₁ Clone # 132016
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	8807 (Human); 16174 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Soluble interleukin-18 receptor complex is a novel biomarker in rheumatoid arthritis. Authors: Takei S, Hoshino T, Matsunaga K, Sakazaki Y, Sawada M, Oda H, Takenaka S, Imaoka H, Kinoshita T, Honda S, Ida H, Fukuda TA, Aizawa H Arthritis Res. Ther., 2011;13(2):R52. Species: Human Sample Type: Serum Application: WB CD molecules 2006--human cell differentiation molecules. Authors: Zola H, Swart B, Banham A, Barry S, Beare A, Bensussan A, Boumsell L, D Buckley C, Buhring HJ, Clark G, Engel P, Fox D, Jin BQ, Macardle PJ, Malavasi F, Mason D, Stockinger H, Yang X J. Immunol. Methods, 2007;319(1):1-5. Species: Human Sample Type: Whole Cells Application: Flow IL-18 receptor beta-induced changes in the presentation of IL-18 binding sites affect ligand binding and signal transduction. Authors: Wu C, Sakorafas P, Miller R, McCarthy D, Scesney S, Dixon R, Ghayur T J. Immunol., 2003;170(11):5571-7. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Mouse myeloma cell line NS0-derived recombinant human IL‑18 R beta /IL‑1 R7 Met1-Arg356 Accession # O95256
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	IL-18, originally described as an interferon-gamma inducing factor (IGIF), is a member of the IL-1 family of cytokines that has multiple immunoregulatory functions. It has potent IFN-gamma inducing activities and plays a key role in the activation of T helper type 1 (Th1) responses. The functional IL-18 receptor complex consists of two components, the IL-18 R alpha (IL-1 R5) and IL-18 R beta (also termed IL-1 R7 and AcPL) subunits. Both subunits are members of the IL-1 receptor superfamily. Although IL‑18 R alpha by itself binds IL-18 with low-affinity and IL-18 R beta does not bind IL-18 in vitro, co-expression of IL-18 R alpha and IL-18 R beta is required for high-affinity binding and IL-18 responsiveness. Human IL-18 R beta cDNA encodes a 599 amino acid (aa) residue precursor type I membrane protein with a 14 aa signal peptide, a 342 aa extracellular region containing three immunoglobulin-like domains, a single transmembrane domain and a 222 aa cytoplasmic domain. Human and mouse IL-18 R beta share 65% aa sequence identity. The expression of IL-18 R beta parallels that of IL-18 R alpha and is detected in numerous tissues including lung, spleen, leukocytes and colon.
运输条件	Blue Ice
存放说明	4℃
参考文献	Born, T.L.et al. (1998) J. Biol. Chem. 273:29445. Okamura, H.et al. (2000) in Cytokine Reference, Vol.2:1605, Academic Press.

参考图片

IFN-gamma secretion Induced by
IL-18/IL1F4 and Neutralization by Human IL-18 R beta /IL-1 R7 Antibody. In the presence of Recombinant Human TNF-alpha (20 ng/mL, Catalog # 210-TA ), Recombinant Human
IL-18/ IL-1F4 stimulates IFN-gamma secretion in the KG-1 human acute myelogenous leukemia cell line in a dose-dependent manner (left-hand graph), as measured by the Human IFN-gamma Quantikine ELISA Kit (Catalog # DIF50). Under these conditions, IFN-gamma secretion elicited by Recombinant Human IL-18/ IL-1F4 (40 ng/mL) is neutralized (right-hand graph) by increasing concentrations of Mouse Anti-Human IL-18 R beta /IL-1 R7 Monoclonal Antibody (Catalog # MAB1181). The ND₅₀ is typically 0.3-1.0 μg/mL.