Mouse M-CSF MAb (Clone 131614) (25 UG)【科瑞奥博】

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Mouse M-CSF MAb (Clone 131614) (25 UG)

货号： MAB4161-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB4161-SP
别名	colony stimulating factor 1 (macrophage); CSF1; CSF-1; Lanimostim; macrophage colony stimulating factor; macrophage colony-stimulating factor 1; M-CSF; MCSFlanimostim; MGC31930
全称	Macrophage Colony Stimulating Factor
反应种属	Mouse
应用	Western Blot,Neutralization
目标/特异性	Detects mouse M-CSF in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human M-CSF or recombinant mouse SCF is observed.
使用方法	Western Blot: 1 µg/mL Neutralization: Measured by its ability to neutralize M‑CSF-induced proliferation in the M‑NFS‑60 mouse myelogenous luekemia lymphoblast cell line. Halenbeck, R. et al. (1989) Biotechnology 7:710. The Neutralization Dose (ND₅₀) is typically 3-12 µg/mL in the presence of 10 ng/mL Recombinant Mouse M‑CSF.
来源	Monoclonal Rat IgG_2B Clone # 131614
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	1435 (Human); 12977 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. CSF-1 receptor-mediated differentiation of a new type of monocytic cell with B cell-stimulating activity: its selective dependence on IL-34. Authors: Yamane F, Nishikawa Y, Matsui K, Asakura M, Iwasaki E, Watanabe K, Tanimoto H, Sano H, Fujiwara Y, Stanley E, Kanayama N, Mabbott N, Magari M, Ohmori H J Leukoc Biol, 2014;95(1):19-31. Species: Mouse Sample Type: Cell Lysate Application: WB Colony-stimulating factor 1 receptor (CSF1R) signaling in injured neurons facilitates protection and survival. Authors: Luo, Jian, Elwood, Fiona, Britschgi, Markus, Villeda, Saul, Zhang, Hui, Ding, Zhaoqing, Zhu, Liyin, Alabsi, Haitham, Getachew, Ruth, Narasimhan, Ramya, Wabl, Rafael, Fainberg, Nina, James, Michelle, Wong, Gordon, Relton, Jane, Gambhir, Sanjiv S, Pollard, Jeffrey, Wyss-Coray, Tony J Exp Med, 2013;210(1):157-72. Species: Mouse Sample Type: tissue Application: IHC Fresh Osteoblasts support B-lymphocyte commitment and differentiation from hematopoietic stem cells. Authors: Zhu J, Garrett R, Jung Y, Zhang Y, Kim N, Wang J, Joe GJ, Hexner E, Choi Y, Taichman RS, Emerson SG Blood, 2007;109(9):3706-12. Species: Mouse Sample Type: Whole Cells Application: Neut
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant mouse M-CSF Lys33-Glu262 (predicted) Accession # P07141
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	M-CSF, also known as CSF-1, is a four-alpha -helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation (1-3). M-CSF is also essential for the survival and proliferation of osteoclast progenitors (1, 4). M-CSF also primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis (2, 3). M-CSF increases during pregnancy to support implantation and growth of the decidua and placenta (5). Sources of M-CSF include fibroblasts, activated macrophages, endometrial secretory epithelium, bone marrow stromal cells, and activated endothelial cells (1-5). The M-CSF receptor (c-fms) transduces its pleotropic effects and mediates its endocytosis. M-CSF mRNAs of various sizes occur (3-9). Full length mouse M-CSF transcripts encode a 520 amino acid (aa) type I transmembrane (TM) protein with a 462 aa extracellular region, a 21 aa TM domain, and a 37 aa cytoplasmic tail that forms a 140 kDa covalent dimer. Differential processing produces two proteolytically cleaved, secreted dimers. One is an N- and O-glycosylated 86 kDa dimer, while the other is modified by both glycosylation and chondroitin-sulfate proteoglycan (PG) to generate a 200 kDa subunit. Although PG-modified M-CSF can circulate, it may be immobilized by attachment to type V collagen (8). Shorter transcripts encode M-CSF that lacks cleavage and PG sites and produces an N-glycosylated 68 kDa TM dimer and a slowly produced 44 kDa secreted dimer (7). Although forms may vary in activity and half-life, all contain the N-terminal 150 aa portion that is necessary and sufficient for interaction with the M-CSF receptor (10, 11). The first 229 aa of mature mouse M-CSF shares 87%, 83%, 82%, and 81% aa identity with corresponding regions of rat, dog, cow, and human M-CSF, respectively (12, 13). Human M-CSF is active in the mouse, but mouse M-CSF is reported to be species-specific.
运输条件	Blue Ice
存放说明	4℃
参考文献	Pixley, F.J. and E.R. Stanley (2004) Trends Cell Biol. 14:628. Chitu, V. and E.R. Stanley (2006) Curr. Opin. Immunol. 18:39. Fixe, P. and V. Praloran (1997) Eur. Cytokine Netw. 8:125. Ryan, G.R. et al. (2001) Blood 98:74. Makrigiannakis, A. et al. (2006) Trends Endocrinol. Metab. 17:178. Nandi, S. et al. (2006) Blood 107:786. Rettenmier, C.W. and M.F. Roussel (1988) Mol. Cell Biol. 8:5026. Suzu, S. et al. (1992) J. Biol. Chem. 267:16812. Manos, M.M. (1988) Mol. Cell. Biol. 8:5035. Koths, K. (1997) Mol. Reprod. Dev. 46:31. Jang, M-H. et al. (2006) J. Immunol. 177:4055. DeLamarter, J.F. et al. (1987) Nucleic Acids Res. 15:2389. Ladner, M.B. et al. (1988) Proc. Natl. Acad. Sci. USA 85:6706.

参考图片

Cell Proliferation Induced by M‑CSF and Neutralization by Mouse M‑CSF Antibody. Recombinant Mouse M‑CSF (Catalog # 416-ML) stimulates proliferation in the M‑NFS‑60 mouse myelogenous luekemia lymphoblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse M‑CSF (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse M‑CSF Monoclonal Antibody (Catalog # MAB4161). The ND₅₀ is typically 3-12 µg/mL.