| 货号 | MAB9496-SP |
| 简介 | Recombinant Monoclonal Antibody |
| 别名 | 5-HT6; 5-hydroxytryptamine (serotonin) receptor 6,5-HT65-HT6R; HTR6; Serotonin receptor 6,5-HT-6,5-hydroxytryptamine receptor 6 | 全称 | 5-Hydroxytryptamine Receptor 6 |
| 应用 | Immunohistochemistry |
| 目标/特异性 | Detects human 5-HT6 in direct ELISAs. |
| 使用方法 | Immunohistochemistry: 1-25 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3362 (Human); 15565 (Mouse); 64354 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Human 5-HT6 peptide Accession # P50406 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | 5-HT6 is a seven transmembrane G-protein coupled 1 receptor for serotonin, with 93% homology between human and rat, and with highest expression in neurons of the striatum, cortex and hippocampus. Expression in cultured primary astrocytes from rats and glia has also been demonstrated for most brain regions. Expression in lymph node, spleen and testis has also been observed. High affinity for psychotropic drugs including tricyclic antidepressants and antipsychotics and high expression in the limbic regions suggest a role for 5-HT6 in emotional behaviors and mental illness. Lower expression of 5-HT6 in cortical layers I-V have been observe in Alzheimers Disease brains. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
5-HT6 in Rat Brain. 5-HT6 was detected in perfusion fixed frozen sections of rat brain (medulla) using Rabbit Anti-Human 5-HT6 Monoclonal Antibody (Catalog # MAB9496) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm of neuronal cell bodies. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections. |
