| 货号 | MAB9455-SP |
| 简介 | Recombinant Monoclonal Antibody |
| 别名 | CREB regulated transcription coactivator 3; CRTC3; FLJ21868; TORC3; TORC-3; TORC3CREB-regulated transcription coactivator 3; Transducer of CREB protein 3; transducer of regulated cAMP response element-binding protein (CREB) 3; Transducer of regulated cAMP response element-binding protein 3; transducer of regulated CREB protein 3 | 全称 | Transducer of Regulated CREB-binding Protein 3 |
| 应用 | Immunocytochemistry |
| 目标/特异性 | Detects human TORC3 in direct ELISAs. |
| 使用方法 | Immunocytochemistry: 1-10 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 64784 (Human); 70461 (Mouse); 365297 (Rat) |
| 纯化方式 | Protein A or G purified from cell culture supernatant |
| 免疫原 | E. coli-derived recombinant human TORC3 Ser425-Asn574 Accession # Q6UUV7 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | CREB-Regulated Transcription Coactivator 3 (CRTC3), alsoknown as TORC3, a transcriptional coactivator for CREB1, acts as a coactivatorin the SIK/TORC signaling pathway. Dephosphorylated CRTC3 increases theinteraction of CREB1 cAMP response element (CRE) sites. CRTC3 activity isimportant in regulating the expression of genes involved in cellular energymetabolism. Its binding to the HTLV-1 protein Tax enhances HTLV-1transcription, whereas its binding to cellular Bcl3 inhibits HTLV-1transcription. CRTC3 polymorphism havebeen associated with obesity and hypertriglyceridemia. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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TORC3 in HeLa Human Cell Line. TORC3 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rabbit Anti-Human TORC3 Monoclonal Antibody (Catalog # MAB9455) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
