Human EGF Neutralizing (D8A1) Rabbit mAb
货号：
12157S 基本售价：
4382.0 元 规格：
-
产品信息
概述| 货号 | 12157S |
| 描述 | Neutralizing antibodies can be used to inhibit normal biological function through their binding to biological molecules. These reagents can be used to determine the effects that a particular molecule has in biological systems. Human EGF Neutralizing (D8A1) Rabbit mAb has been shown to neutralize the EGF-induced proliferation of MCF 10A cells in vitro with an ND50 in the range of 200-700 ng/ml. |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | N |
| 目标/特异性 | Human EGF Neutralizing (D8A1) Rabbit mAb binds to human EGF and neutralizes its effects in an MCF 10A cell proliferation assay. This antibody does not cross-react with human betacellulin or human TGF-α. |
| 使用方法 | N(1:1) |
性能| 供应商 | CST |
| 背景 | EGF is produced by epithelial cells, fibroblasts, and many other cell types (1,2). Low molecular weight soluble EGF is generated through proteolysis of a larger ~130,000 kDa transmembrane precursor (1,2). Both soluble and membrane forms of EGF are active (2). EGF induces proliferation, differentiation, and survival of many cell types including tumor-derived cells (1-3). There are multiple members of the EGF family and multiple members of the HER/ErbB EGF receptor family. EGF binds to HER1/ErbB1 and induces homo- or heterodimerization with other HER/ErbB family members, resulting in signaling through the MAPK, PI3K/Akt, and Stat5 pathways (1). Research studies have implicated EGF, EGF family members, EGF receptors, and their signaling pathways in many cancers (1,2). |
| 存放说明 | -20C |
参考图片The proliferation of MCF 10A cells treated with increasing concentrations of hEGF #8916 was assessed. After 24 hr, cells were labeled with BrdU for 4 hr and BrdU incorporation was determined using BrdU Cell Proliferation Assay Kit #6813.MCF 10A细胞的增殖通过递增浓度的hEGF #8916处理进行评估。24小时后，用BrdU标记细胞4小时，BrdU的结合用 BrdU Cell Proliferation Assay Kit #6813测定。 |
The ability of Human EGF Neutralizing (D8A1) Rabbit mAb to inhibit hEGF-induced MCF 10A cell proliferation was assessed. MCF 10A cells were treated with increasing concentrations of antibody in the presence of hEGF #8916 (2 ng/ml). After 24 hr, cells were labeled with BrdU for 4 hr and BrdU incorporation was determined using BrdU Cell Proliferation Assay Kit #6813.Human EGF Neutralizing (D8A1) Rabbit mAb能够抑制hEGF诱导的MCF 10A细胞的增殖。MCF 10A细胞通过hEGF #8916 (2 ng/ml)浓度抗体处理。24小时后，细胞被用BrdU标记4小时，BrdU的结合用 BrdU Cell Proliferation Assay Kit #6813测定。 |
