Human IL-4 Neutralizing (D20H1) Rabbit mAb
货号：
11964S 基本售价：
4382.0 元 规格：
-
产品信息
概述| 货号 | 11964S |
| 描述 | Neutralizing antibodies can be used to inhibit normal biological function through their binding to biological molecules. These reagents can be used to determine the effects that a particular molecule has in biological systems. Human IL-4 Neutralizing (D20H1) Rabbit mAb has been shown to neutralize the proliferation of TF-1 cells in vitro with an ND50 in the range of 3-19 ng/ml. |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | N |
| 目标/特异性 | Human IL-4 Neutralizing (D20H1) Rabbit mAb binds to human IL-4 (hIL-4) and neutralizes its effects in a TF-1 cell proliferation assay. This antibody does not cross-react with mouse IL-4, human IL-13, or mouse IL-13. |
| 使用方法 | N(1:1) |
性能| 供应商 | CST |
| 背景 | Interleukin-4 (IL-4) is produced by T cells, NK T cells, γδ cells, and mast cells (1). Target cells include B cells, T cells, and macrophages (1). IL-4 induces differentiation of naive T cells into the Th2 phenotype. IL-4 also promotes B cell proliferation, antibody isotype switching, and expression of other Th2 cytokines including IL-5 and IL-9. IL-4-induced Th2 polarization is important in developing humoral immunity against extracellular pathogens (1) and is involved in the development of allergy and asthma (2). IL-4 binds to two distinct receptors, the type I receptor and type II receptor. The type I receptor is a heterodimer consisting of IL-4Rα chain and the common gamma chain, γc (3,4). The type II receptor, which is shared with IL-13, is a heterodimer of IL-4Rα and IL-13Rα1. Signaling initiated via type I receptor results in the activation of Jak1/Stat6, Jak3, and the PI3K/Akt pathways (3). The type II receptor activates the Jak1/Stat6 and the Tyk2/Stat3 pathways (3). |
| 存放说明 | -20C |
参考图片The proliferation of TF-1 cells treated with increasing concentrations of hIL-4 #8919 was assessed. After 72 hr, cells were incubated with a tetrazolium salt and the OD450 was determined.使用递增浓度的hIL-4 #8919处理的TF-1细胞的增殖已被评估。72小时后，细胞使用四唑盐处理，并在OD450处检测。 |
The ability of Human IL-4 Neutralizing (D20H1) Rabbit mAb to inhibit hIL-4-induced TF-1 cell proliferation was assessed. Cells were incubated with increasing concentrations of antibody in the presence of hIL-4 #8919 (500 pg/ml). After 72 hr, viable cells were detected by incubation with a tetrazolium salt and the OD450 was determined.Human IL-4 Neutralizing (D20H1) Rabbit mAb具有抑制hIL-4诱导的TF-1细胞增殖的能力已被评估。细胞与浓度递增的抗体hIL-4 #8919 (500 pg/ml)共同孵育。72小时后，活细胞通过四唑盐的处理，在OD450进行检测。 |
