Human TNF-α Neutralizing (D1B4) Rabbit mAb
货号：
7321S 基本售价：
4382.0 元 规格：
-
产品信息
概述| 货号 | 7321S |
| 描述 | Neutralizing antibodies can be used to inhibit normal biological function through their binding to biological molecules. These reagents can be used to determine the effects that a particular molecule has in biological systems. TNF-α has known functions of cell cytotoxicity, cell activation, and apoptosis in different cell types. Human TNF-α Neutralizing (D1B4) Rabbit mAb has been shown to neutralize the cytotoxic effects of TNF-α in L-929 mouse fibroblast cells. Utilizing 1 ng/ml of hTNF-α #8902 and 1 µg/ml of actinomycin D, Human TNF-α Neutralizing (D1B4) Rabbit mAb rescued L-929 cells with an ND50 in the range of 4-12 ng/ml. |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | N |
| 目标/特异性 | Human TNF-α Neutralizing (D1B4) Rabbit mAb binds to human TNF-α and neutralizes its cytotoxic effects. This antibody does not cross-react with mouse TNF-α. |
| 使用方法 | N(1:1) |
性能| 供应商 | CST |
| 背景 | TNF-α, the prototypical member of the TNF protein superfamily, is a homotrimeric type-II membrane protein (1,2). Membrane-bound TNF-α is cleaved by the metalloprotease TACE/ADAM17 to generate a soluble homotrimer (2). Both membrane and soluble forms of TNF-α are biologically active. TNF-α is produced by a variety of immune cells including T cells, B cells, NK cells, and macrophages (1). Cellular response to TNF-α is mediated through interaction with receptors TNF-R1 and TNF-R2 and results in activation of pathways that favor both cell survival and apoptosis depending on the cell type and biological context. Activation of kinase pathways (including JNK, Erk (p44/42), p38 MAPK, and NF-κB) promotes the survival of cells, while TNF-α-mediated activation of caspase-8 leads to programmed cell death (1,2). TNF-α plays a key regulatory role in inflammation and host defense against bacterial infection, notably Mycobacterium tuberculosis (3). The role of TNF-α in autoimmunity is underscored by blocking TNF-α action to treat rheumatoid arthritis and Crohn’s disease (1,2,4). |
| 存放说明 | -20C |
参考图片The viability of L-929 cells, treated with hTNF-α #8902, in the presence of 1 μg/ml actinomycin D was determined. After 24 hour treatment with hTNF-α, viable cells were stained with a tetrazolium salt and the OD450 was determined.L-929细胞的生存能力，在1 μg/ml放射菌素D 存在下用hTNF-α #8902处理。24 hr后用hTNF-α处理, 存活细胞用四唑盐染色并测定OD450数值。 |
The neutralization ability of Human TNF-α Neutralizing (D1B4) Rabbit mAb on hTNF-α-induced cell cytotoxicity was assessed by adding increasing concentrations of antibody with 1 ng/ml of hTNF-α #8902, before addition to L-929 cells in the presence of 1 μg/ml actinomycin D. After 24 hour incubation, viable cells were stained and % neutralization was calculated.Human TNF-α Neutralizing (D1B4) Rabbit mAb的抑制能力是通过加入递增浓度的抗体和1 ng/ml hTNF-α #8902到经hTNF-α诱导的细胞毒性的L-929细胞中（在1 μg/ml放射菌素D 存在下）。孵育24 hr后,存活的细胞染色并计算抑制率。 |
