Mouse TNF-α Neutralizing (D2H4) Rabbit mAb
货号：
11969S 基本售价：
4382.0 元 规格：
-
产品信息
概述| 货号 | 11969S |
| 描述 | Neutralizing antibodies can be used to inhibit normal biological function through their binding to biological molecules. These reagents can be used to determine the effects that a particular molecule has in biological systems. Mouse TNF-α Neutralizing (D2H4) Rabbit mAb has been shown to neutralize the cytotoxic effects of TNF-α in L-929 cells in vitro with an ND50 in the range of 1-6 ng/ml. |
| 反应种属 | Mouse |
| 来源宿主 | Rabbit |
| 应用 | N |
| 目标/特异性 | Mouse TNF-α Neutralizing (D2H4) Rabbit mAb binds to mouse TNF-α and neutralizes its cytotoxic effects. This antibody does not cross-react with human TNF-α or human LTA. |
| 使用方法 | N(1:1) |
性能| 供应商 | CST |
| 背景 | TNF-α, the prototypical member of the TNF protein superfamily, is a homotrimeric type-II membrane protein (1,2). Membrane-bound TNF-α is cleaved by the metalloprotease TACE/ADAM17 to generate a soluble homotrimer (2). Both membrane and soluble forms of TNF-α are biologically active. TNF-α is produced by a variety of immune cells including T cells, B cells, NK cells, and macrophages (1). Cellular response to TNF-α is mediated through interaction with receptors TNF-R1 and TNF-R2, and results in activation of pathways that favor both cell survival and apoptosis depending on the cell type and biological context. Activation of kinase pathways (including JNK, Erk (p44/42), p38 MAPK, and NF-κB) promotes the survival of cells, while TNF-α-mediated activation of caspase-8 leads to programmed cell death (1,2). TNF-α plays a key regulatory role in inflammation and host defense against bacterial infection, notably Mycobacterium tuberculosis (3). The role of TNF-α in autoimmunity is underscored by research studies that show that blocking TNF-α action may be used to treat rheumatoid arthritis and Crohn’s disease (1,2,4). |
| 存放说明 | -20C |
参考图片The viability of L-929 cells treated with increasing amounts of mTNF-α #5178 in the presence of 1 µg/ml actinomycin D was determined. After a 24 hr treatment, cells were incubated with a tetrazolium salt and the OD450 was determined.使用含有1 µg/ml 放线菌素D的浓度递增的mTNF-α #5178处理L-929细胞，并检查生存活性。处理24小时后，细胞中加入四唑盐，在OD450处检测。 |
The ability of Mouse TNF-α Neutralizing (D2H4) Rabbit mAb to inhibit mTNF-α-induced L-929 cell cytotoxicity was assessed. Cells were incubated with increasing concentrations of antibody in the presence of mTNF-α #5178 (250 pg/ml) and 1 µg/ml actinomycin D. After 24 hr, viable cells were detected by incubation with a tetrazolium salt and the OD450 was determined.Mouse TNF-α Neutralizing (D2H4) Rabbit mAb具有抑制mTNF-α诱导的L-929细胞毒性的能力已被评估。细胞与浓度递增的抗体（含有mTNF-α #5178 (250 pg/ml) 和1 µg/ml actinomycin D）共同孵育。24小时后，活细胞通过四唑盐的处理，在OD450进行检测。 |
