| 货号 | 41039S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 应用 | F |
| 目标/特异性 | ALK (D5F3®) XP® Rabbit mAb (Alexa Fluor® 700 Conjugate) detects endogenous levels of total ALK protein as well as ALK fusion proteins, such as EML4-ALK variants and NPM-ALK, even at low levels. This antibody does not cross-react with other family members. |
| 使用方法 | Flow Cytometry (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Anaplastic lymphoma kinase (ALK) is a tyrosine kinase receptor for pleiotrophin (PTN), a growth factor involved in embryonic brain development (1-3). In ALK-expressing cells, PTN induces phosphorylation of both ALK and the downstream effectors IRS-1, Shc, PLCγ, and PI3 kinase (1). ALK was originally discovered as a nucleophosmin (NPM)-ALK fusion protein produced by a translocation (4). Investigators have found that the NPM-ALK fusion protein is a constitutively active, oncogenic tyrosine kinase associated with anaplastic lymphoma (4). Research literature suggests that activation of PLCγ by NPM-ALK may be a crucial step for its mitogenic activity and involved in the pathogenesis of anaplastic lymphomas (5). A distinct ALK oncogenic fusion protein involving ALK and echinoderm microtubule-associated protein like 4 (EML4) has been described in the research literature from a non-small cell lung cancer (NSCLC) cell line, with corresponding fusion transcripts present in some cases of lung adenocarcinoma. The short, amino-terminal region of the microtubule-associated protein EML4 is fused to the kinase domain of ALK (6-8). |
| 存放说明 | 4C |
| 参考文献 | 1 . Stoica, G.E. et al. (2001) J Biol Chem 276, 16772-9. 2 . Iwahara, T. et al. (1997) Oncogene 14, 439-49. 3 . Morris, S.W. et al. (1997) Oncogene 14, 2175-88. 4 . Morris, S.W. et al. (1994) Science 263, 1281-4. 5 . Bai, R.Y. et al. (1998) Mol Cell Biol 18, 6951-61. 6 . Rikova, K. et al. (2007) Cell 131, 1190-203. 7 . Takeuchi, K. et al. (2008) Clin Cancer Res 14, 6618-24. 8 . Soda, M. et al. (2007) Nature 448, 561-6. |
Flow cytometric analysis of TF-1 cells (blue) and KARPAS-299 cells (green) using ALK (D5F3®) XP® Rabbit mAb (Alexa Fluor® 700 Conjugate). Cell Line Source: Dr Abraham Karpas at the University of Cambridge. |
