| 货号 | 6952S |
| 描述 | This Cell Signaling Technology® antibody is conjugated by the covalent reaction of hydrazinonicotinamide-modified antibody with formylbenzamide-modified horseradish peroxidase (HRP). The HRP conjugated antibody is expected to exhibit the same species cross-reactivity as the unconjugated Acetylated-Lysine (Ac-K2-100) Rabbit mAb #9814. |
| 反应种属 | Human/Mouse/Monkey/All |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Acetylated-Lysine (Ac-K2-100) Rabbit mAb (HRP Conjugate) detects proteins post-translationally modified by acetylation on the ε-amine groups of lysine residues. The antibody recognizes acetylated lysine in a wide range of sequence contexts. It has been demonstrated to recognize acetylated histones, p53, CBP, PCAF, and chemically acetylated BSA. The antibody has been shown to react with as little as 0.04 ng of chemically acetylated BSA while not recognizing up to 25 µg of non-acetylated BSA. (U.S. Patent Nos.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.) |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 标记 | HRP |
| 背景 | Acetylation of lysine, like phosphorylation of serine, threonine or tyrosine, is an important reversible modification controlling protein activity. The conserved amino-terminal domains of the four core histones (H2A, H2B, H3, and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (1). Signaling resulting in acetylation/deacetylation of histones, transcription factors, and other proteins affects a diverse array of cellular processes including chromatin structure and gene activity, cell growth, differentiation, and apoptosis (2-6). Recent proteomic surveys suggest that acetylation of lysine residues may be a widespread and important form of posttranslational protein modification that affects thousands of proteins involved in control of cell cycle and metabolism, longevity, actin polymerization, and nuclear transport (7,8). The regulation of protein acetylation status is impaired in cancer and polyglutamine diseases (9), and HDACs have become promising targets for anti-cancer drugs currently in development (10). |
| 存放说明 | -20C |
Western blot analysis of extracts from MCF7 cells, untreated or treated with Trichostatin A (TSA) #9950 (1 µM, 6 hr), using Acetylated-Lysine (Ac-K2-100) Rabbit mAb (HRP Conjugate). 对未处理或1uM Trichostatin A (TSA) #9950处理6小时的MCF7细胞抽提液使用Acetylated-Lysine (Ac-K2-100) Rabbit mAb (HRP Conjugate)进行Western blot分析。 | |
Signal-to-noise (S/N) values of acetylated versus non-acetylated peptides analyzed using Acetylated-Lysine (Ac-K2-100) Rabbit mAb (HRP Conjugate) and 20X LumiGLO® Reagent and 20X Peroxide #7003. 使用Acetylated-Lysine (Ac-K2-100) Rabbit mAb (HRP Conjugate)和20X LumiGLO® Reagent和20X Peroxide #7003得到的乙酰化肽段和非乙酰化肽段的信噪比。 |
