EGF Receptor (EGFR1) Mouse mAb (Sepharose®Bead Conjugate)
货号：
8083S 基本售价：
4060.0 元 规格：
-
产品信息
概述| 货号 | 8083S |
| 描述 | This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated sepharose beads. EGF Receptor (EGFR1) Mouse mAb (IP Specific) (Sepharose Bead Conjugate) is useful for immunoprecipitation assays. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated EGF Receptor (EGFR1) Mouse mAb (IP Specific) #2256. |
| 反应种属 | Human |
| 来源宿主 | Mouse |
| 应用 | IP |
| 目标/特异性 | EGF Receptor (EGFR1) Mouse mAb (IP Specific) (Sepharose Bead Conjugate) specifically immunoprecipitates endogenous EGFR1 protein from various cell lysates. This antibody does not cross-react with other EGF receptor family members. |
| 使用方法 | IP(1:20) |
性能| 供应商 | CST |
| 标记 | Sepharose Bead |
| 背景 | The epidermal growth factor (EGF) receptor is a transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Ligand binding results in receptor dimerization, autophosphorylation, activation of downstream signaling, internalization, and lysosomal degradation (1,2). Phosphorylation of EGF receptor (EGFR) at Tyr845 in the kinase domain is implicated in stabilizing the activation loop, maintaining the active state enzyme, and providing a binding surface for substrate proteins (3,4). c-Src is involved in phosphorylation of EGFR at Tyr845 (5). The SH2 domain of PLCγ binds at phospho-Tyr992, resulting in activation of PLCγ-mediated downstream signaling (6). Phosphorylation of EGFR at Tyr1045 creates a major docking site for the adaptor protein c-Cbl, leading to receptor ubiquitination and degradation following EGFR activation (7,8). The GRB2 adaptor protein binds activated EGFR at phospho-Tyr1068 (9). A pair of phosphorylated EGFR residues (Tyr1148 and Tyr1173) provide a docking site for the Shc scaffold protein, with both sites involved in MAP kinase signaling activation (2). Phosphorylation of EGFR at specific serine and threonine residues attenuates EGFR kinase activity. EGFR carboxy-terminal residues Ser1046 and Ser1047 are phosphorylated by CaM kinase II; mutation of either of these serines results in upregulated EGFR tyrosine autophosphorylation (10). |
| 存放说明 | -20C |
| 计算分子量 | 175 |
参考图片Immunoprecipitation of HeLa cell lysates using Mouse IgG (Sepharose Bead Conjugate) #3420 (lane 1) and EGF Receptor (EGFR1) Mouse mAb (IP Specific) (Sepharose Bead Conjugate) (lane 2). The western blot was probed using EGF Receptor (D38B1) XP® Rabbit mAb #4267.免疫沉淀方法检测HeLa细胞裂解物，使用的抗体为Mouse IgG (Sepharose Bead Conjugate) #3420 (泳道1)和EGF Receptor (EGFR1) Mouse mAb (IP Specific) (琼脂糖珠结合) (泳道2). 探索的western blot使用的抗体为EGF Receptor (D38B1) XP® Rabbit mAb #4267. |
