| 货号 | 8814S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-IC/F/ChIP |
| 使用方法 | WB(1:1000) IP (1:200) IHC-P (1:800) F (1:800) IF-IC (1:400) ChIP (1:100) |
| 供应商 | CST |
| 背景 | β-catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3 (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8). |
| 存放说明 | -20C |
| 计算分子量 | 92 |
Western blot analysis of extracts from 293T cells, untreated (-) or treated (+) with LiCl (20 mM, 20 hr at 37ºC), using Non-phospho (Active) β-Catenin (Ser33/37/Thr41) (D13A1) Rabbit mAb (left) and β-Catenin Antibody #9562 (right). Equal protein loading was confirmed using β-Tubulin (9F3) Rabbit mAb #2128. | |
Non-phospho (Active) β-Catenin (Ser33/37/Thr41) (D13A1) Rabbit mAb specificity was determined by peptide competition ELISA. Graph depicts binding of the antibody to non-phospho-β-catenin (Ser33/37/Thr41) peptide in the presence of increasing concentrations of competitor peptides. As shown, non-phospho-β-catenin peptides compete for binding, whereas tri-phospho-β-catenin (Ser33/37/Thr41) peptide does not compete for binding. | |
Western blot analysis of extracts from 293T cells, untreated (-) or treated (+) with MG132 (10 μM, 4 hr at 37ºC), using Non-phospho (Active) β-Catenin (Ser33/37/Thr41) (D13A1) Rabbit mAb (left), Phospho-β-Catenin (Ser33/37/Thr41) Antibody #9561 (center), or β-Catenin (6B3) Rabbit mAb #9582 (right). Note that Non-phospho (Active) β-Catenin (Ser33/37/Thr41) (D13A1) Rabbit mAb fails to detect poly-ubiquitinated β-catenin in MG132-treated cells, indicating its specificity for stabilized protein. | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HCT 116 cells and either 5 μl of Non-phospho (Active) β-Catenin (Ser33/37/Thr41) (D13A1) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human Axin2 Intron 1 Primers #8973, SimpleChIP® Human CaMK2D Intron 3 Primers #5111, human c-Myc promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. | |
Immunohistochemical analysis of paraffin embedded cell pellets, HeLa (left) or NCI-H28 (right), using Non-phospho (Active) β-Catenin (Ser33/37/Thr41) (D13A1) Rabbit mAb. | |
Immunohistochemical analysis of paraffin embedded mouse colon using Non-phospho (Active) β-Catenin (Ser33/37/Thr41) (D13A1) Rabbit mAb in the presence of phospho-β-catenin (Ser33/37/Thr41) peptide (left) or non-phospho-β-catenin (Ser33/37/Thr41) peptide (right). Note the absence of staining only in the presence of the non-phospho-β-catenin (Ser33/37/Thr41) peptide, demonstrating non-phospho specificity. | |
Immunohistochemical analysis of paraffin embedded human breast carcinoma using Non-phospho (Active) β-Catenin (Ser33/37/Thr41) (D13A1) Rabbit mAb. | |
Flow cytometric analysis of K562 cells, untreated (blue) or treated with 6-bromoindirubin-3-oxime (BIO) (30 nM, 40 hr) (green) using Non-phospho (Active) β-Catenin (Ser33/37/Thr41) (D13A1) Rabbit mAb. | |
Confocal immunofluorescent analysis of HCT-15 (left, center; positive) or NCI-H28 (right; negative) cells using Non-phospho (Active) β-Catenin (Ser33/37/Thr41) (D13A1) Rabbit mAb (green) in the presence of Phospho-Catenin-β (Ser33/Ser37/Thr41) peptide (left) or Non-phospho-Catenin-β (Ser33/Ser37/Thr41) peptide (center). Red = Propidium Iodide (PI)/RNase Staining Solution #4087. | |
Immunohistochemical analysis of paraffin-embedded Apc (Min/+) mouse intestinal adenoma (left) and adjacent normal intestinal epithelium (right) using Non-phospho (Active) beta-Catenin (Ser33/37/Thr41) (D13A1) Rabbit mAb. Note the nuclear accumulation of active beta-Catenin in the adenoma cells. |
