| 货号 | 12178S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Phospho-Akt (Thr450) (D5G4) Rabbit mAb recognizes endogenous levels of Akt1 only when phosphorylated at Thr450. This antibody also recognizes Akt2 and Akt3 when phosphorylated at the corresponding residues. It does not recognize Akt phosphorylated at other sites. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis (1-3). This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase (2,3). Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4) and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1 (5,6). Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including Bad (7), forkhead transcription factors (8), c-Raf (9), and caspase-9. PTEN phosphatase is a major negative regulator of the PI3 kinase/Akt signaling pathway (10). LY294002 is a specific PI3 kinase inhibitor (11). Another essential Akt function is the regulation of glycogen synthesis through phosphorylation and inactivation of GSK-3α and β (12,13). Akt may also play a role in insulin stimulation of glucose transport (12). In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regulation by preventing GSK-3β-mediated phosphorylation and degradation of cyclin D1 (14) and by negatively regulating the cyclin dependent kinase inhibitors p27 Kip1 (15) and p21 Waf1/Cip1 (16). Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor (17). More importantly, Akt phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex (18,19). JNK reactivates Akt after ischemic injury by phosphorylating Thr450, a priming event for subsequent phosphorylation by 3-phosphoinositide-dependent protein kinase (20). |
| 存放说明 | -20C |
| 计算分子量 | 60 |
Western blot analysis of extracts from LNCaP cells, untreated, serum-starved, or Torin-treated as indicated, using Phospho-Akt (Thr450) (D5G4) Rabbit mAb (upper) or Akt (pan) (C67E7) Rabbit mAb #4691 (lower). 未处理，血清饥饿，或Torin处理的LNCaP细胞提取物，使用Phospho-Akt (Thr450) (D5G4)Rabbit mAb兔单抗（上）或Akt（pan）(C67E7)Rabbit mAb兔单抗#4691（下）进行western blot分析。 | |
Western blot analysis of extracts from various cells lines using Phospho-Akt (Thr450) (D5G4) Rabbit mAb (upper) or Akt (pan) (C67E7) Rabbit mAb #4691 (lower). 使用Phospho-Akt (Thr450) (D5G4)Rabbit mAb兔单抗（上）或Akt（pan）(C67E7)Rabbit mAb兔单抗#4691（下）对多种细胞系提取物进行western blot分析。 | |
Immunoprecipitation of Phopho-Akt (Thr450) from MEF cell extracts, using Normal Rabbit IgG #2729 (lane 2) or Phospho-Akt (Thr450) (D5G4) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Phospho-Akt (Thr450) (D5G4) Rabbit mAb. 使用正常Rabbit IgG #2729 (lane 2)或Phospho-Akt (Thr450) (D5G4) Rabbit mAb 兔单抗(lane 3)对MEF细胞提取物中的Phopho-Akt (Thr450)进行免疫沉淀实验。Lane1是10%上样量。Phospho-Akt (Thr450) (D5G4)Rabbit mAb兔单抗用于western blot分析。 |
