| 货号 | 6986S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Phospho-APP (Thr668) (D90B8) Rabbit mAb detects different isoforms of endogenous amyloid β (A4) precursor protein only when phosphorylated at Thr668 (or the corresponding position of other isoforms). |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Amyloid β (A4) precursor protein (APP) is a 100-140 kDa transmembrane glycoprotein that exists as several isoforms (1). The amino acid sequence of APP contains the amyloid domain (A-β), which can be released by a two-step proteolytic cleavage (1). The extracellular deposition and accumulation of the released A-β fragments form the main components of amyloid plaques in Alzheimer disease (1). APP can be phosphorylated at several sites, which may affect the proteolytic processing and secretion of this protein (2-5). The phosphorylation at Thr668 (at a position corresponding to the APP695 isoform) by cyclin-dependent kinase is cell cycle dependent and peaks during G2/M-phase (4). The APP Thr668 phosphorylated form exists in adult rat brain and correlates with cultured neuronal differentiation (5,6). |
| 存放说明 | -20C |
| 计算分子量 | 100-140 |
Western blot analysis of extracts from HeLa cells, untreated or nocodazole-treated (1 μg/ml, 18 hr) using Phospho-APP (Thr668) (D90B8) Rabbit mAb (upper) or APP Antibody #2452 (lower). Western blot分析HeLa细胞（未处理或nocodazole处理，1μg/ml，18小时），使用的抗体是Phospho-APP (Thr668) (D90B8) Rabbit mAb 兔单抗（上图）或APP Antibody 兔多抗 #2452（下图）。 |
