货号 | 13346S |
反应种属 | Human/Mouse/Rat |
来源宿主 | Mouse |
应用 | W/IP |
使用方法 | WB(1:1000) IP (1:100) |
供应商 | CST |
背景 | Transcription factors of the nuclear factor κB (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which translocates to the nucleus (9-11). |
存放说明 | -20C |
计算分子量 | 65 |
Western blot analysis of extracts from HeLa and PC-3 cells, untreated (-) or treated with Human Tumor Necrosis Factor-α (hTNF-α) #8902 (hTNF-α; 20 ng/ml, 5 min; +), using Phospho-NF-κB p65 (Ser536) (E1Z1T) Mouse mAb (upper) or NF-κB p65 (D14E12) XP® Rabbit mAb #8242 (lower). | |
Western blot analysis of extracts from THP-1 cells differentiated with TPA #4174 (80 nM, overnight), untreated (-) or LPS-treated (1 μg/ml, 15 min; +), using Phospho-NF-κB p65 (Ser536) (E1Z1T) Mouse mAb (upper) or NF-κB p65 (D14E12) XP® Rabbit mAb #8242 (lower). | |
Immunoprecipitation of phospho-NF-κB p65 (Ser536) from HeLa cells treated with Human Tumor Necrosis Factor-α (hTNF-α) #8902 (20 ng/ml, 5 min) using Mouse (G3A1) mAb IgG1 Isotype Control #5415 (lane 2) or Phospho-NF-κB p65 (Ser536) (E1Z1T) Mouse mAb (lane 3). Lane 1 represents 10% input. Western blot was performed using Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb #3033. |