| 货号 | 9878S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 目标/特异性 | Phospho-Numb (Ser276) (D5C2) Rabbit mAb recognizes endogenous levels of numb protein only when phosphorylated at Ser276. |
| 使用方法 | WB(1:1000) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | Numb contains an amino-terminal phosphotyrosine-binding (PTB) domain and carboxy-terminal endocytic binding motifs for α-adaptin and EH (Eps15 homology) domain-containing proteins, indicating a role in endocytosis (1,2). There are four mammalian Numb splicing isoforms that are differentially expressed and may have distinct functions (3-5). Numb acts as a negative regulator of Notch signaling by promoting ubiquitination and degradation of Notch (6). The protein is asymmetrically segregated into one daughter cell during cell division, producing two daughter cells with different responses to Notch signaling and different cell fates (7,8). The localization of Numb can also be regulated by G-protein coupled receptor (GPCR) and PKC signaling (9). |
| 存放说明 | -20C |
| 计算分子量 | 78 |
Western blot analysis of extracts from HeLa and U-87 MG cells, untreated or treated with either λ phosphatase or TPA #4174 (200 nM, 30 min), using Phospho-Numb (Ser276) (D5C2) Rabbit mAb (upper) or Numb (C29G11) Rabbit mAb #2756 (lower). | |
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with either TPA #4174 (center) or TPA and λ phosphatase (right), using Phospho-Numb (Ser276) (D5C2) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
