Phospho-Nur77 (Ser351) (D22G5) Rabbit mAb
货号：
5095S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 5095S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | Nur77, also known as TR3 and NGFI-B, is an immediate-early response gene and an orphan member of the steroid/thyroid/retinoid receptor superfamily (1-3). Nur77 is composed of an amino-terminal transactivation domain, a central DNA-binding domain and a carboxy-terminal ligand-binding domain. Expression of Nur77 is rapidly induced by a variety of stimuli, including apoptotic, mitogenic and stress signals (1-6). It has been proposed to have many functions related to cell proliferation, differentiation and apoptosis. Nur77 has been extensively studied in T cells where it has been implicated in the process of negative selection and TCR-mediated apoptosis (5,6). Nur77 binds to specific DNA elements leading to the regulation of target genes (7). As a possible mechanism for regulating apoptosis, Nur77 can induce the expression of apoptotic genes such as FasL and TRAIL (8,9). Nur77 is heavily phosphorylated by multiple kinases, which may affect its transactivation activity as well as its subcellular localization (4,10,11). Translocation of Nur77 from the nucleus to the mitochondria can regulate its association with Bcl-2 and control the release of cytochrome c, thereby triggering apoptosis (12,13).Nur77也称为TR3和NGFI-B，是一种即早反应基因和类固醇/甲状腺/类视黄醇受体超家族的孤儿成员（1-3）。 Nur77由一个氨基末端反式激活域，一个中央DNA结合结构域和一个羧基-末端配体结合域组成。Nur77的表达由多种刺激快速诱导，包括细胞凋亡、有丝分裂和应激信号（1-6）。已经提出有多个与细胞增殖、分化和细胞凋亡相关的功能。在T细胞中，Nur77已被广泛研究，它与负选择过程和TCR介导的细胞凋亡有关（5,6）。Nur77结合到特定的DNA分子，导致靶基因调控（7）。作为一种调节细胞凋亡的可能机制，Nur77可以诱导凋亡基因的表达，如FasL和TRAIL（8,9）。 Nur77很大程度上是由多种激酶磷酸化，从而可能影响其转录活性以及其亚细胞定位（4,10,11）。Nur77从细胞核易位到线粒体，可以调节其与Bcl-2的联合，并控制细胞色素c的释放，从而引发细胞凋亡（12,13）。 |
| 存放说明 | -20C |
| 计算分子量 | 70-80 |
参考图片Western blot analysis of extracts from Jurkat cells, untreated or treated with TPA #4174 and calcium ionophore A23187 for the indicated times, using Phospho-Nur77 (Ser351) (D22G5) Rabbit mAb (upper) or Nur77 (D63C5) XP® Rabbit mAb #3960 (lower).Western blot方法检测Jurkat细胞提取物，细胞不处理或用TPA #4174和钙离子载体按指定的时间处理，使用的抗体为Phospho-Nur77 (Ser351) (D22G5) Rabbit mAb (上图)或 Nur77 (D63C5) XP® Rabbit mAb #3960 (下图). |
Western blot analysis of extracts from Jurkat cells, untreated or treated with TPA #4174 and calcium ionophore A23187 for 4 hours, using Phospho-Nur77 (Ser351) (D22G5) Rabbit mAb. Antibody phospho-specificity was confirmed by pre-incubating the antibody with either a phosphorylated site-specific peptide to block the signal or a non-phosphorylated site-specific peptide that does not block the signal.Western blot方法检测Jurkat细胞提取物，细胞不处理或用TPA #4174和钙离子载体处理4小时，使用的抗体为Phospho-Nur77 (Ser351) (D22G5) Rabbit mAb. 通过将抗体与磷酸化位点特异性多肽进行预孵育以阻断信号，或非磷酸化的位点特异性多肽不阻断信号，证实了抗体磷酸化的特异性。 |
