| 货号 | 11989S |
| 反应种属 | Human/Mouse/Rat/Monkey/Mink |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IF-IC |
| 使用方法 | WB(1:1000) IHC-P (1:300) IF-IC (1:800) |
| 供应商 | CST |
| 背景 | The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed Ser/Thr kinases characterized by two nonidentical, functional kinase domains (1) and a carboxy-terminal docking site for extracellular signal-regulated kinases (ERKs) (2). Several sites both within and outside of the RSK kinase domain, including Ser380, Thr359, Ser363, and Thr573, are important for kinase activation (3). RSK1-3 are activated via coordinated phosphorylation by MAPKs, autophosphorylation, and phosphoinositide-3-OH kinase (PI3K) in response to many growth factors, polypeptide hormones, and neurotransmitters (3). |
| 存放说明 | -20C |
| 计算分子量 | 90 |
Confocal immunofluorescent analysis of NIH/3T3 cells, serum-starved (left), treated with TPA #4174 (200 nM, 15 min; center), or treated with TPA followed by λ phosphatase (right), using Phospho-p90RSK (Ser380) (D3H11) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).血清饥饿（左），使用TPA #4174 (200 nM, 15 min; 中)处理,或TPA后再加入λ phosphatase（右）处理的NIH/3T3细胞，使用Phospho-p90RSK (Ser380) (D3H11)Rabbit mAb（绿色）进行激光共聚焦免疫荧光分析。肌动蛋白丝使用DY-554鬼笔环肽标记（红色）。蓝色假色= DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from various cell lines, starved overnight and either untreated (-) or treated (+) with TPA #4174 (200 nM, 15 min), Human Epidermal Growth Factor (hEGF) #8916 (100 ng/mL, 15 min), or Human Platelet-Derived Growth Factor BB (hPDGF-BB) #8912 (100 ng/mL, 15 min) as indicated, using Phospho-p90RSK (Ser380) (D3H11) Rabbit mAb.血清饥饿过夜然后不处理（-）或用TPA #4174 (200 nM, 15 min)及Human Epidermal Growth Factor (hEGF) #8916 (100 ng/mL, 15 min)处理（+），或TPA #4174 (200 nM, 15 min)及Human Platelet-Derived Growth Factor BB (hPDGF-BB) #8912 (100 ng/mL, 15 min)处理的多种细胞提取物进行，使用Phospho-p90RSK (Ser380) (D3H11)Rabbit mAb进行western blot分析。 | |
Immunohistochemical analysis of paraffin-embedded HeLa cell pellets, untreated (left) or treated with TPA #4174 (right), using Phospho-p90RSK (Ser380) (D3H11) Rabbit mAb.未处理（左）或TPA #4174（右）处理的石蜡包埋HeLa细胞，使用Phospho-p90RSK (Ser380) (D3H11)Rabbit mAb进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using Phospho-p90RSK (Ser380) (D3H11) Rabbit mAb.使用Phospho-p90RSK (Ser380) (D3H11)Rabbit mAb对石蜡包埋的人卵巢癌组织进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, control (left) or λ phosphatase-treated (right), using Phospho-p90RSK (Ser380) (D3H11) Rabbit mAb.对照（左）或λ phosphatase处理（右）的石蜡包埋的人结肠癌组织，使用Phospho-p90RSK (Ser380) (D3H11)Rabbit mAb进行免疫组化分析。 |
