| 货号 | 24188S |
| 反应种属 | Human |
| 来源宿主 | Rabbit IgG |
| 应用 | W/IP |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | Platelet derived growth factor (PDGF) family proteins exist as several disulphide-bonded, dimeric isoforms (PDGF AA, PDGF AB, PDGF BB, PDGF CC, and PDGF DD) that bind in a specific pattern to two closely related receptor tyrosine kinases, PDGF receptor α (PDGFRα) and PDGF receptor β (PDGFRβ). PDGFRα and PDGFRβ share 75% to 85% sequence homology between their two intracellular kinase domains, while the kinase insert and carboxy-terminal tail regions display a lower level (27% to 28%) of homology (1). PDGFRα homodimers bind all PDGF isoforms except those containing PDGF D. PDGFRβ homodimers bind PDGF BB and DD isoforms, as well as the PDGF AB heterodimer. The heteromeric PDGF receptor α/β binds PDGF B, C, and D homodimers, as well as the PDGF AB heterodimer (2). PDGFRα and PDGFRβ can each form heterodimers with EGFR, which is also activated by PDGF (3). Various cells differ in the total number of receptors present and in the receptor subunit composition, which may account for responsive differences among cell types to PDGF binding (4). Ligand binding induces receptor dimerization and autophosphorylation, followed by binding and activation of cytoplasmic SH2 domain-containing signal transduction molecules, such as GRB2, Src, GAP, PI3 kinase, PLCγ, and NCK. A number of different signaling pathways are initiated by activated PDGF receptors and lead to control of cell growth, actin reorganization, migration, and differentiation (5). |
| 存放说明 | -20C |
| 计算分子量 | 190 |
Immunoprecipitation of Phospho-PDGF Receptor α (Tyr762) protein from Human Platelet-Derived Growth Factor AA (hPDGF-AA) #8913 treated (100 ng/ml, 5 min) NCI-H1703 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Phospho-PDGF Receptor α (Tyr762) (D9B1N) Rabbit mAb. Western blot analysis was performed using Phospho-PDGF Receptor α (Tyr762) (D9B1N) Rabbit mAb (multiple partially degraded phospho-PDGF Receptor α bands shown at lower molecular size). | |
Western blot analysis of extracts from NCI-H1703 cells serum-starved overnight and then either untreated (-) or treated with Human Platelet-Derived Growth Factor AA (hPDGF-AA) #8913 (100 ng/ml, 10 min;+), using Phospho-PDGF Receptor α (Tyr762) Rabbit mAb (upper) or PDGF Receptor α (D13C6) XP® Rabbit mAb #5241 (lower). |
