Phospho-Smad1 (Ser463/465)/ Smad5 (Ser463/465)/ Smad9 (Ser465/467) (D5B10) Rabbit mAb (ChIP formulated)
货号：
11971S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 11971S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | ChIP |
| 目标/特异性 | Phospho-Smad1/5 (Ser463/465) (D5B10) Rabbit mAb (ChIP formulated) recognizes endogenous levels of Smad1 and Smad5 protein when phosphorylated at Ser463/465. |
| 使用方法 | ChIP(1:100) |
性能| 供应商 | CST |
| 背景 | Bone morphogenetic proteins (BMPs) constitute a large family of signaling molecules that regulate a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation, and apoptosis (1,2). BMP receptors are members of the TGF-β family of Ser/Thr kinase receptors. Ligand binding induces multimerization, autophosphorylation, and activation of these receptors (3-5). They subsequently phosphorylate Smad1 at Ser463 and Ser465 in the carboxy-terminal motif SSXS, as well as Smad5 and Smad8 at their corresponding sites. These phosphorylated Smads dimerize with the coactivating Smad4 and translocate to the nucleus, where they stimulate transcription of target genes (5). |
| 存放说明 | -20C |
参考图片Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 MCF7 cells treated with Human BMP2 #4697 (50 ng/ml) for 1 hr and either 5 μl of Phospho-Smad1/5 (Ser463/465) (D5B10) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIp® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human ID1 Promoter Primers #5139, human SMAD6 promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. 使用SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003将Human BMP2 #4697 (50 ng/ml)处理1小时的4 x 106 MCF7细胞与5μl的Phospho-Smad1/5 (Ser463/465) (D5B10) Rabbit mAb或2μl的Normal Rabbit IgG #2729交联然后进行染色质免疫共沉淀实验。富集的DNA使用SimpleChIP® Human ID1 Promoter 引物 #5139 human SMAD6 promoter 引物，和SimpleChIP® Human α Satellite Repeat 引物 #4486以real-time PCR的方式定量。各样品沉淀得到的DNA量相对于input染色质总量进行相对定量，Input中染色质量设定为1。 |
