| 货号 | 8826S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/ChIP |
| 目标/特异性 | Phospho-Stat1 (Ser727) (D3B7) Rabbit mAb recognizes endogenous levels of Stat1 protein only when phosphorylated at Ser727. |
| 使用方法 | WB(1:1000) IP (1:100) IHC-P (1:800) ChIP (1:50) |
| 供应商 | CST |
| 背景 | The Stat1 transcription factor is activated in response to a large number of ligands (1) and is essential for responsiveness to IFN-α and IFN-γ (2,3). Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation, and DNA binding (4). Stat1 protein exists as a pair of isoforms, Stat1α (91 kDa) and the splice variant Stat1β (84 kDa). In most cells, both isoforms are activated by IFN-α, but only Stat1α is activated by IFN-γ. The inappropriate activation of Stat1 occurs in many tumors (5). In addition to tyrosine phosphorylation, Stat1 is also phosphorylated at Ser727 through a p38 mitogen-activated protein kinase (MAPK)-dependent pathway in response to IFN-α and other cellular stresses (6). Serine phosphorylation may be required for the maximal induction of Stat1-mediated gene activation. |
| 存放说明 | -20C |
| 计算分子量 | 91 |
Immunohistochemical analysis of paraffin-embedded human lymphoma, control (left) or λ phosphatase-treated (right) using Phospho-Stat1 (Ser727) (D3B7) Rabbit mAb.对石蜡包埋的人淋巴瘤(对照)(左图)或 λ磷酸化处理的(右图)进行免疫组化分析，使用抗体是Phospho-Stat1 (Ser727) (D3B7) Rabbit mAb。 | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HT-1080 cells treated with IFN-γ (50 ng/ml) for 30 minutes and either 10 μl of Phospho-Stat1 (Ser727) (D3B7) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human IRF-1 promoter primers, SimpleChIP® Human TAP1 Promoter Primers #5148, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.染色质免疫沉淀法检测4 x 106 HT-1080细胞中的交联染色质，细胞通过IFN-γ (50 ng/ml)处理30分钟或者使用 10 μl Phospho-Stat1 (Ser727) (D3B7) Rabbit mAb 或 2 μl of Normal Rabbit IgG #2729处理。使用试剂盒是SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003。丰富的DNA通过使用human IRF-1 promoter primers, SimpleChIP® Human TAP1 Promoter Primers #5148, 和SimpleChIP® Human α Satellite Repeat Primers #4486引物进行Real-Time PCR来量化。每个样品中免疫沉淀得到的DNA总量与投入的核酸总量相当。 | |
Western blot analysis of extracts from various cell lines, untreated (-) or UV-treated (2 hr recovery; +), using Phospho-Stat1 (Ser727) (D3B7) Rabbit mAb.Western blot分析多种细胞系的细胞提取物，未处理(-)或UV处理(2 hr 复原; +)，使用抗体是Phospho-Stat1 (Ser727) (D3B7) Rabbit mAb。 | |
Western blot analysis of extracts from UV-treated HeLa cells, untreated (-) or CIP and λ phosphatase-treated (+), using Phospho-Stat1 (Ser727) (D3B7) Rabbit mAb (upper) or Stat1 (42H3) Rabbit mAb #9175 (lower).Western blot分析UV处理的HeLa细胞的细胞提取物，未处理(-)或CIP和 λ磷酸化处理(+)，使用抗体是Phospho-Stat1 (Ser727) (D3B7) Rabbit mAb (上图) 或 Stat1 (42H3) Rabbit mAb #9175 (下图)。 | |
Western blot analysis of extracts from HeLa cells, untreated (-) or treated with Human Interferon-γ (hIFN-γ) #8901 (100 ng/ml, 30 min; +), using Phospho-Stat1 (Ser727) (D3B7) Rabbit mAb (upper) or Stat1 (42H3) Rabbit mAb #9175 (lower).Western blot分析HeLa细胞的细胞提取物，未处理(-)或 Human Interferon-γ (hIFN-γ) #8901 (100 ng/ml, 30 分钟; +)处理, 使用抗体是Phospho-Stat1 (Ser727) (D3B7) Rabbit mAb (上图) 或 Stat1 (42H3) Rabbit mAb #9175 (下图)。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Phospho-Stat1 (Ser727) (D3B7) Rabbit mAb.对石蜡包埋的人乳腺癌组织进行免疫组化分析，使用抗体是Phospho-Stat1 (Ser727) (D3B7) Rabbit mAb。 | |
Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using Phospho-Stat1 (Ser727) (D3B7) Rabbit mAb.对石蜡包埋的卵巢癌组织进行免疫组化分析，使用抗体是Phospho-Stat1 (Ser727) (D3B7) Rabbit mAb。 |
