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货号： bs-23810R-Bio 基本售价： 2980.0 元 规格： 100ul
产品信息
- 产品编号
- bs-23810R-Bio
- 英文名称
- Anti-Histone H4/Biotin
- 中文名称
- 生物素标记的组蛋白H4抗体
- 别 名
- H4F2; H4FN; HIST1H4; HIST2H4; HISTH4H4; methyl histone H4; histone H4; H4_HUMAN; Osteogenic growth peptide; OGP.
- 规格价格
- 100ul/2980元购买 大包装/询价
- 说 明 书
- 100ul
- 研究领域
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat,
- 产品应用
- WB=1:50-200 ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200 ICC=1:50-200 IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 11kDa
- 性 状
- Lyophilized or Liquid
- 浓 度
- 2mg/1ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human Histone H4
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- 产品介绍
- background:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. [provided by RefSeq, Jul 2008].
Function:
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Subunit:
The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
Subcellular Location:
Nucleus. Chromosome.
Post-translational modifications:
Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
Phosphorylated by PAK2 at Ser-48 (H4S47ph). This phosphorylation increases the association of H3.3-H4 with the histone chaperone HIRA, thus promoting nucleosome assembly of H3.3-H4 and inhibiting nucleosome assembly of H3.1-H4.
Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
Sumoylated, which is associated with transcriptional repression.
Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes.
Similarity:
Belongs to the histone H4 family.
Database links:Entrez Gene: 121504Human
Entrez Gene: 554313Human
Entrez Gene: 8294Human
Entrez Gene: 8359Human
Entrez Gene: 8360Human
Entrez Gene: 8361Human
Entrez Gene: 8362Human
Entrez Gene: 8363Human
Entrez Gene: 8364Human
Entrez Gene: 8365Human
Entrez Gene: 8366Human
Entrez Gene: 8367Human
Entrez Gene: 8368Human
Entrez Gene: 8370Human
Entrez Gene: 100041230Mouse
Entrez Gene: 100862646Mouse
Entrez Gene: 319155Mouse
Entrez Gene: 319156Mouse
Entrez Gene: 319157Mouse
Entrez Gene: 319158Mouse
Entrez Gene: 319159Mouse
Entrez Gene: 319160Mouse
Entrez Gene: 319161Mouse
Entrez Gene: 320332Mouse
Entrez Gene: 326619Mouse
Entrez Gene: 326620Mouse
Entrez Gene: 69386Mouse
Entrez Gene: 97122Mouse
GenBank: NM_003548Human
Omim: 142750Human
SwissProt: P02304Human
SwissProt: P62805Human
SwissProt: P02304Mouse
SwissProt: P62806Mouse
SwissProt: P02304Rat
Unigene: 143080Human
Unigene: 247816Human
Unigene: 248172Human
Unigene: 248178Human
Unigene: 248179Human
Unigene: 278483Human
Unigene: 352191Human
Unigene: 46423Human
Unigene: 528055Human
Unigene: 533295Human
Unigene: 55468Human
Unigene: 591790Human
Unigene: 655235Human
Unigene: 662174Human
Unigene: 706635Human
Unigene: 742244Human
Unigene: 14775Mouse
Unigene: 158272Mouse
Unigene: 227295Mouse
Unigene: 228709Mouse
Unigene: 246720Mouse
Unigene: 255646Mouse
Unigene: 260530Mouse
Unigene: 261642Mouse
Unigene: 261662Mouse
Unigene: 261664Mouse
Unigene: 377875Mouse
Unigene: 442307Mouse
Unigene: 486099Mouse
Unigene: 489077Mouse
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.