Nanog (D73G4) XP® Rabbit mAb (ChIP Formulated)
货号：
5232S 基本售价：
3860.0 元 规格：
-
产品信息
概述| 货号 | 5232S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | ChIP |
| 使用方法 | ChIP(1:50) |
性能| 供应商 | CST |
| 背景 | Nanog is a homeodomain-containing transcription factor that is essential for the maintenance of pluripotency and self renewal in embryonic stem cells (1). Nanog expression is controlled by a network of factors including Sox2 and the key pluripotency regulator Oct-4 (1). Recent advances in somatic cell reprogramming have utilized viral expression of combinations of transcription factors including nanog, Oct-4, Sox2, KLF4, c-Myc, and Lin28 (2,3). Nanog是一类同源结构域包含转录因子，为胚胎干细胞多潜能性和自我更新所必需（1） Nanog的表达受Sox2、多潜能性调节关键因子Oct-4（1）。最近关于体细胞程序化重编的进展用到了包括nanog, Oct-4, Sox2, KLF4, c-Myc, and Lin28在内的转录因子协同的病毒表达（2,3）。 |
| 存放说明 | -20C |
参考图片Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 NCCIT cells and either 10 μl of Nanog (D73G4) XP® Rabbit mAb (ChIP Formulated) or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human Oct-4 Promoter Primers #4641, SimpleChIP® Human Sox2 Promoter Primers #4649, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.4 x 106 NCCIT细胞细胞染色质与SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003的10μl Nanog (D73G4) XP®兔源单克隆抗体（ChIP级别）或2μl正常兔IgG#2729 交联。富集到的DNA随后使用SimpleChIP® Human Oct-4启动子引物#4641，SimpleChIP® Human Sox2启动子引物#4649和SimpleChIP®人α卫星重复序列引物#4486，进行荧光实时PCR定量。各样品沉淀得到的DNA量通过与input染色质总量对比获得，相当于1份.4 x 106 NTERA-2细胞。 |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 NTERA-2 cells and 10 μl of Nanog, Oct-4 and Sox2 antibodies, or 2 μl of Normal Rabbit IgG, using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human Nanog promoter primers, SimpleChIP® Human Oct-4 Promoter Primers #4641, SimpleChIP® Human Sox2 Promoter Primers #4649, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.使用SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003将4 x 106 NCCIT细胞染色质与10μl Nanog, Oct-4和Sox2抗体或2 μl正常兔IgG交联。富集到的DNA随后使用人Nanog启动子引物，SimpleChIP®人Oct-4启动子引物#4641，SimpleChIP®人Sox2启动子引物#4649和SimpleChIP®人α卫星重复序列引物#4486进行荧光实时PCR定量。各样品沉淀得到的DNA量通过与input染色质总量对比获得，相当于1份.4 x 106 NTERA-2细胞。 |
