| 货号 | 3608S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/ChIP |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:400) ChIP (1:100) |
| 供应商 | CST |
| 背景 | Notch1 is a transmembrane protein functioning in development and the determination of cell fate (1). During maturation, the notch molecule is cleaved by a furin-like convertase at its extracellular domain (2). Upon binding to a ligand such as Delta1, or upon extracellular calcium depletion, the carboxy-terminal notch1 fragment is released and further cleaved between Gly1743 and Val1744 (3,4). The resulting activated cytosolic fragment translocates to the nucleus where it activates transcription. |
| 存放说明 | -20C |
| 计算分子量 | 120, 300 |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Notch1 (D1E11) XP® Rabbit mAb. 使用Notch1 (D1E11) XP® Rabbit mAb兔单抗对石蜡包埋的人乳腺癌组织进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded human inflammatory granulation tissue using Notch1 (D1E11) XP® Rabbit mAb. 使用Notch1 (D1E11) XP®兔单抗对石蜡包埋的人炎症肉芽组织进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded A2780 (left), Jurkat (center) and RL (right) cell pellets using Notch1 (D1E11) XP® Rabbit mAb. Both A2780 and Jurkat express Notch1, but only Jurkat cells have cleaved Notch1, while RL cells express very low or no Notch1. 使用Notch1 (D1E11) XP® Rabbit mAb兔单抗对石蜡包埋的A2780（左），Jurkat（中）和RL（右）细胞进行免疫组化分析。A2780和Jurkat表达Notch1，但仅有Jurkat表达Notch1的剪切型，而RL细胞中Notch1表达量极低或不表达。 | |
Western blot analysis of total cell extract from various cell types using Notch1 (D1E11) XP® Rabbit mAb. The full-length (FL) Notch protein and the cleaved transmembrane/intracellular region (NTM) are indicated. 使用Notch1 (D1E11) XP® Rabbit mAb兔单抗对多种细胞提取物进行western blot分析。Notch1的全长（FL）蛋白和剪切后跨膜/细胞内部位（NTM）如图所示。 | |
Immunohistochemical analysis of paraffin-embedded human colon using Notch1 (D1E11) XP® Rabbit mAb. 使用Notch1 (D1E11) XP® Rabbit mAb兔单抗对石蜡包埋的人结肠组织进行免疫组化分析。 |
