Sox2 (D6D9) XP® Rabbit mAb (ChIP Formulated)
货号：
5024S 基本售价：
3860.0 元 规格：
-
产品信息
概述| 货号 | 5024S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | ChIP |
| 使用方法 | ChIP(1:50) |
性能| 供应商 | CST |
| 背景 | Embryonic stem cells are derived from the inner cell mass of the blastocyst and are unique in their pluripotent capacity and potential for self-renewal. Sox2 is one of a set of transcription factors that are crucial for the maintenance of pluripotency (1). Sox2, Oct-4, and Nanog cooperate in this network (1-3) and siRNA knockdown of either Sox2 or Oct-4 results in loss of pluripotency (4,5). Chromatin immunoprecipitation experiments have shown that Sox2 and Oct-4 bind to thousands of gene regulatory sites, highlighting the importance of these transcription factors in early embryonic development (6,7). It has recently been shown that Sox2 is amplified in lung and esophageal squamous cell tumors (8). |
| 存放说明 | -20C |
参考图片Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 NCCIT cells and either 10 μl of Sox2 (D6D9) XP® Rabbit mAb (ChIP Formulated) or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human Oct-4 Promoter Primers #4641, SimpleChIP® Human Sox2 Promoter Primers #4649, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.4 x 106 NCCIT细胞染色质与SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003的10μl Sox2 (D6D9) XP®兔源单克隆抗体（ChIP级别）或2μl 正常兔源IgG#2729交联。富集到的DNA使用SimpleChIP® Human Oct-4启动子引物#4641，SimpleChIP® 人Sox2启动子引物#4649和SimpleChIP®人α卫星重复引物#4486进行荧光实时PCR定量。各样品沉淀得到的DNA量通过与input染色质总量对比获得，相当于1份.4 x 106 NTERA-2细胞。 |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 NTERA-2 cells and either 10 μl of Nanog (D73G4) XP® Rabbit mAb (ChIP Formulated) #5232, Oct-4A (C30A3C1) Rabbit mAb (ChIP Formulated) #5677, Sox2 (D6D9) XP® Rabbit mAb (ChIP Formulated), or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human Nanog promoter primers, SimpleChIP® Human Oct-4 Promoter Primers #4641, SimpleChIP® Human Sox2 Promoter Primers #4649, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.NTERA-2细胞染色质与SimpleChIP® Enzymatic Chromatin IP 试剂盒(Magnetic Beads) #9003 10 μl Nanog (D73G4) XP®兔源单克隆抗体(ChIP 级别) #5232, Oct-4A (C30A3C1)兔源单克隆抗体(ChIP 级别) #5677, Sox2 (D6D9) XP® 兔源单克隆抗体（ChIP 级别），或2 μl 正常兔源IgG #2729，进行染色质免疫共沉淀。富集到的DNA使用人Nanog启动子引物，SimpleChIP® Human Oct-4启动子引物#4641，SimpleChIP® 人Nanog Sox2启动子引物#4649和SimpleChIP®人α卫星重复引物#4486进行荧光实时PCR定量。各样品沉淀得到的DNA量通过与input染色质总量对比获得，相当于1份.4 x 106 NTERA-2细胞。 |
