| 货号 | 5147S |
| 反应种属 | Human/Mouse/Rat/Monkey/Pig |
| 来源宿主 | Rabbit |
| 应用 | W/IP/ChIP |
| 使用方法 | WB(1:1000) IP (1:50) ChIP (1:100) |
| 供应商 | CST |
| 背景 | The serum response factor (SRF) is a 67 kDa phospho-protein that, together with auxiliary factors, modulates transcription of immediate early genes containing serum response elements at their promoters (1,2). SRF contains several phosphorylation sites (3), but functional consequences of phosphorylation have not been identified unequivocally. Several growth factor- and calcium-regulated kinases, such as p90RSK and CaM kinase IV, can phosphorylate SRF at Ser103 (4,5), and Ser103 of SRF is also a nuclear target for MAPKAP kinase 2 (6). |
| 存放说明 | -20C |
| 计算分子量 | 67 |
Western blot analysis of extracts from various cell lines using SRF (D71A9) XP® Rabbit mAb.western blot方法检测细胞提取物：各种细胞系，使用的抗体是SRF (D71A9) XP® Rabbit mAb。 | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HeLa cells starved overnight and treated with TPA (12-O-Tetradecanoylphorbol-13-Acetate) #4174 (100 ng/ml) for 3 hours, and either 5 μl of SRF1 (D71A9) XP® Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human c-Fos Promoter Primers #4663 and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.染色质免疫沉淀反应中使用的是无培养基过夜和TPA (12-O-Tetradecanoylphorbol-13-Acetate) #4174 (100 ng/ml)处理3 h后的4 x 106HeLa细胞中交联的染色质，使用的抗体是5 μl SRF1 (D71A9) XP® Rabbit mAb和2 μl Normal Rabbit IgG #2729，试剂盒是SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003。real-time PCR定量DNA的量，使用的引物是SimpleChIP® Human c-Fos Promoter Primers #4663和SimpleChIP® Human α Satellite Repeat Primers #4486。每个样品中免疫沉淀DNA的量相当于加入总染色质的量。 |
