| 货号 | 8768S |
| 反应种属 | Human/Mouse/Rat/Hamster/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-IC/ChIP |
| 目标/特异性 | Stat3α (D1A5) XP® Rabbit mAb recognizes endogenous levels of total Stat3α protein. This antibody does not cross-react with Stat3β. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:500) IF-IC (1:200) ChIP (1:100) |
| 供应商 | CST |
| 背景 | The Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors (1) and is required for murine fetal development (2). Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure, or cell maturation stage (10). It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8). |
| 存放说明 | -20C |
| 计算分子量 | 86 |
Confocal immunofluorescent analysis of HeLa cells, serum-starved (left) or treated with Human Interferon-α1 (hIFN-α1) #8927 (100 ng/mL, 30 min; right) using Stat3α (D1A5) XP® Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red).共聚焦分析HeLa细胞，血清饥饿(左图)或Human Interferon-α1 (hIFN-α1) #8927 (100 ng/mL, 30 分钟; 右图)处理，使用抗体是Stat3α (D1A5) XP® Rabbit mAb (绿色) 和 β-Actin (8H10D10) Mouse mAb #3700 (红色)。 | |
Western blot analysis of extracts from various cell lines using Stat3α (D1A5) XP® Rabbit mAb (top), Stat3 (124H6) Mouse mAb #9139 (middle), or β-Actin (D6A8) Rabbit mAb #8457 (lower).Western blot分析多种细胞系的细胞提取物，使用抗体是Stat3α (D1A5) XP® Rabbit mAb (上图), Stat3 (124H6) Mouse mAb #9139 (中图),或β-Actin (D6A8) Rabbit mAb #8457 (下图)。 | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 Hep G2 cells starved overnight and treated with Human Interleukin-6 (hIL-6) #8904 (100 ng/ml, 30 min), and either 5 μl of Stat3α (D1A5) XP® Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human IRF-1 promoter primers, SimpleChIP® Human c-Fos Promoter Primers #4663, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.染色质免疫沉淀法检测4 x 106 HepG2细胞中的交联染色质，细胞通过饥饿过夜或者使用Human Interleukin-6 (hIL-6) #8904 (100 ng/ml, 30 分钟) 或5 μl 的 Stat3α (D1A5) XP® Rabbit mAb或2 μl of Normal Rabbit IgG #2729处理。使用试剂盒是SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003。丰富的DNA通过使用human IRF-1 promoter primers, SimpleChIP® Human c-Fos Promoter Primers #4663,和SimpleChIP® Human α Satellite Repeat Primers #4486引物进行Real-Time PCR来量化。每个样品中免疫沉淀得到的DNA总量与投入的核酸总量相当。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Stat3α (D1A5) XP® Rabbit mAb in the presence of control peptide (left) or antige-specific peptide (right).对石蜡包埋的人结肠癌组织进行免疫组化分析，使用抗体是Stat3α (D1A5) XP® Rabbit mAb含有对照抗原肽(左图) 或者特异性抗原肽(右图)。 | |
Immunohistochemical analysis of paraffin-embedded cell pellets, HeLa (left) or PC-3 (right), using Stat3α (D1A5) XP® Rabbit mAb.对石蜡包埋的细胞颗粒，HeLa(左图)或PC-3(右图)进行免疫组化分析，使用抗体是Stat3α (D1A5) XP® Rabbit mAb。 |
