| 货号 | 12800S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/ChIP |
| 使用方法 | WB(1:1000) IP (1:100) IF-IC (1:100) ChIP (1:50) |
| 供应商 | CST |
| 背景 | Like other nuclear hormone receptors, STF-1 has a modular domain structure composed of an amino-terminal zinc finger DNA-binding domain, a ligand-binding domain, a carboxy-terminal AF-2 activation domain, and a hinge region with AF-1-like activation activity. STF-1 also contains a fushi tarazu factor 1 box, which functions as an accessory DNA binding domain (11). STF-1 is primarily phosphorylated at Ser203, which is thought to enhance its transcriptional activity by promoting complex formation with transcriptional cofactors (12). In addition to phosphorylation at Ser203, STF-1 is subject to SUMO conjugation and acetylation at ε-amino groups of target lysine residues. Whereas SUMOylation represses STF-1 function (13,14), acetylation enhances its transcriptional activity (15). |
| 存放说明 | -20C |
| 计算分子量 | 50 |
Confocal immunofluorescent analysis of NCI-H295R (positive, left) and SW-13 (negative, right) cells using STF-1 (D1Z2A) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). | |
Western blot analysis of extracts from various cell lines using STF-1 (D1Z2A) XP® Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). | |
Immunoprecipitation of STF-1 from NCI-H295R cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or STF-1 (D1Z2A) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using STF-1 (D1Z2A) XP® Rabbit mAb. | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing DDK-tagged full-length human STF-1 (hSTF-1-DDK; +) or Myc/DDK-tagged full-length human LRH-1, isoform 2 (hLRH-1-Myc/DDK; +), using STF1 (D1Z2A) XP® Rabbit mAb (upper) and DYKDDDDK Tag Antibody #2368 (lower). | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 NCI-H295R cells treated with dibutyryl cAMP (0.4mM) for 1h and either 10 µl of STF-1 (D1Z2A) XP®Rabbit mAb or 2 µl of Normal Rabbit IgG #2729, using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human CYP17A1 intron 1 primers, SimpleChIP® Human StAR Intron 1 Primers #12864, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. |
