| 货号 | 3737S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/ChIP |
| 目标/特异性 | SUZ12 (D39F6) XP® Rabbit mAb detects endogenous levels of SUZ12 protein. |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:800) ChIP (1:100) |
| 供应商 | CST |
| 背景 | The polycomb group (PcG) proteins contribute to the maintenance of cell identity, stem cell self-renewal, cell cycle regulation and oncogenesis by maintaining the silenced state of genes that promote cell lineage specification, cell death and cell-cycle arrest (1-4). PcG proteins exist in two complexes that cooperate to maintain long-term gene silencing through epigenetic chromatin modifications. The first complex, EED-EZH2, is recruited to genes by DNA-binding transcription factors and methylates histone H3 on Lys27. Methylation of Lys27 facilitates the recruitment of the second complex, PRC1, which ubiquitinylates histone H2A on Lys119 (5). Suppressor of Zeste 12 (SUZ12) is a component of the PRC2 complex, which together with Ezh2 and Eed is absolutely required for histone methyl-transferase activity (6). SUZ12 contains a C2H2 zinc finger domain similar to the zinc finger domains found in sequence-specific DNA binding proteins and may mediate the interaction between EZH2 and nucleosomes (6). SUZ12 is overexpressed in several human tumors, including tumors of the colon, breast and liver (7,8). |
| 存放说明 | -20C |
| 计算分子量 | 83 |
Western blot analysis of extracts from various cell lines using SUZ12 (D39F6) XP® Rabbit mAb. 使用SUZ12 (D39F6) XP® Rabbit mAb，免疫印迹(Western blot)分析不同细胞中SUZ12 (D39F6)的蛋白水平。 | |
Confocal immunofluorescent analysis of HeLa cells (left) and NTERA2 cells (right) using SUZ12 (D39F6) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). 使用SUZ12 (D39F6) XP® Rabbit mAb (绿色)，共聚焦免疫荧光分析HeLa (左图)和NTERA2细胞。DY-554 phalloidin标记微丝蛋白(红色)。 | |
Confocal immunofluorescent analysis of mouse embryonic stem cells growing on mouse embryonic fibroblast (MEF) feeder cells using SUZ12 (D39F6) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). | |
使用SUZ12 (D39F6) XP® Rabbit mAb (绿色)，共聚焦免疫荧光分析小鼠胚胎干细胞，生长在mouse embryonic fibroblast (MEF)培养层的细胞。DY-554 phalloidin标记微丝蛋白(红色)。 |
