| 货号 | 13232S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-IC/F/ChIP |
| 使用方法 | WB(1:1000) IP (1:100) IHC-P (1:1600) F (1:50) IF-IC (1:400) ChIP (1:50) |
| 供应商 | CST |
| 背景 | The T-box gene family consists of transcription factors characterized by a related DNA-binding domain (T-box) of approximately 200 amino acids (1,2). The T-box genes exhibit diverse temporal and spatial patterns in the developing embryo. Studies have demonstrated members of this family play crucial roles during embryogenesis in a wide range of organisms by regulating cell fate decisions to establish the early body plan and to regulate later processes underlying organogenesis (3-5). Mutations in T-box genes are associated with many developmental defects (6). Recent studies also indicate potential roles in cancer by members of T-box family (7-9). |
| 存放说明 | -20C |
| 计算分子量 | 58-68 |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 NK-92 cells and either 10 μl of T-bet/TBX21 (D6N8B) XP® Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human CCL4 promoter primers, SimpleChIP® Human IFN-γ Promoter Primers #13051, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. | |
Immunohistochemical analysis of paraffin-embedded Non-Hodgkins B-cell lymphoma using T-bet/TBX21 (D6N8B) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using T-bet/TBX21 (D6N8B) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human tonsil using T-bet/TBX21 (D6N8B) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). | |
Western blot analysis of extracts from NK-92 and K-562 cells using T-bet/TBX21 (D6N8B) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Immunofluorescent analysis of NK-92 (positive, left) and K-562 (negative, right) cells using T-bet/TBX21 XP® (D6N8B) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Flow cytometric analysis of human PBMCs using T-bet/TBX21 (D6N8B) XP® Rabbit mAb co-stained with either CD56, CD3 or CD19. CD56+ NK cells and a subset of CD3+ T cells are both distinctly positive for T-bet/TBX21 whereas CD19+ B cells are negative. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 647 Conjugate) #4414 was used as a secondary antibody for the T-Bet/TBX21 primary. |
