| 货号 | 88677S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | H R |
| 来源宿主 | Rabbit IgG |
| 应用 | W IP |
| 目标/特异性 | STEAP1 (D8B2V) Rabbit mAb recognizes endogenous levels of total STEAP1 protein. This antibody also recognizes overexpressed STEAP1B isoform 1 protein and may recognize endogenous STEAP1B isoform 1 protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Six transmembrane epithelial antigen of the prostate 1 (STEAP1) is a transmembrane protein abundantly expressed in normal prostate epithelial cells (1). It is a metalloreductase that reduces Fe3+ to Fe2+ and Cu2+ to Cu1+ (2). STEAP1 is also reportedly overexpressed in multiple tumor types, and has been used as a cell surface biomarker for prostate cancer and Ewings sarcoma (3-6). Peptides derived from STEAP1 protein have been shown to induce an antigen-specific cytotoxic T lymphocyte (CTL) response that can kill tumor cells expressing STEAP1 (7). These finding are the basis for research studies examining the efficacy of antibody-drug conjugates (ADCs) targeting STEAP1 (8,9). |
| 运输条件 | 0.75 |
| 存放说明 | -20C |
| 计算分子量 | 30 |
| 参考文献 | 1 . Hubert, R.S. et al. (1999) Proc Natl Acad Sci U S A 96, 14523-8. 2 . Ohgami, R.S. et al. (2006) Blood 108, 1388-94. 3 . Grunewald, T.G. et al. (2012) Mol Cancer Res 10, 52-65. 4 . Grunewald, T.G. et al. (2012) Ann Oncol 23, 2185-90. 5 . Ihlaseh-Catalano, S.M. et al. (2013) Histopathology 63, 678-85. 6 . Gomes, I.M. et al. (2014) Urol Oncol 32, 53.e23-9. 7 . Rodeberg, D.A. et al. (2005) Clin Cancer Res 11, 4545-52. 8 . Williams, S.P. et al. (2016) Oncotarget 7, 25103-12. 9 . Sukumaran, S. et al. (2016) AAPS J , . |
Western blot analysis of extracts from LnCAP cells and human prostate tissue using STEAP1 (D8B2V) Rabbit mAb. | |
Immunoprecipitation of STEAP1 from LnCap cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is STEAP1 (D8B2V) Rabbit mAb. Western blot analysis was performed using STEAP1 (D8B2V) Rabbit mAb. |
