| 货号 | 18527S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | H M |
| 来源宿主 | Rabbit IgG |
| 应用 | W |
| 目标/特异性 | TRAF4 (D1N3A) Rabbit mAb recognizes endogenous levels of total TRAF4 protein. An unknown background band is detected in some cell lines at 80kDa. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | TRAFs (TNF receptor-associated factors) are a family of multifunctional adaptor proteins that bind to surface receptors and recruit additional proteins to form multiprotein signaling complexes capable of promoting cellular responses (1-3). Members of the TRAF family share a common carboxy-terminal "TRAF domain", which mediates interactions with associated proteins; many also contain amino-terminal Zinc/RING finger motifs. The first TRAFs identified, TRAF1 and TRAF2, were found by virtue of their interactions with the cytoplasmic domain of TNF-receptor 2 (TNFRII) (4). The six known TRAFs (TRAF1-6) act as adaptor proteins for a wide range of cell surface receptors and participate in the regulation of cell survival, proliferation, differentiation, and stress responses. |
| 运输条件 | 0.75 |
| 存放说明 | -20C |
| 计算分子量 | 50 |
| 参考文献 | 1 . Arch, R.H. et al. (1998) Genes Dev 12, 2821-30. 2 . Chung, J.Y. et al. (2002) J Cell Sci 115, 679-88. 3 . Bradley, J.R. and Pober, J.S. (2001) Oncogene 20, 6482-91. 4 . Rothe, M. et al. (1994) Cell 78, 681-92. 5 . Régnier, C.H. et al. (1995) J Biol Chem 270, 25715-21. 6 . Bièche, I. et al. (1996) Cancer Res 56, 3886-90. 7 . Yang, K. et al. (2015) Int J Clin Exp Pathol 8, 1419-26. 8 . Camilleri-Broët, S. et al. (2007) Oncogene 26, 142-7. 9 . Li, W. et al. (2013) Cancer Res 73, 6938-50. 10 . Ye, X. et al. (1999) J Biol Chem 274, 30202-8. 11 . Esparza, E.M. and Arch, R.H. (2004) Cell Mol Life Sci 61, 3087-92. 12 . Krajewska, M. et al. (1998) Am J Pathol 152, 1549-61. 13 . Sax, J.K. and El-Deiry, W.S. (2003) J Biol Chem 278, 36435-44. 14 . Zhang, L. et al. (2013) Mol Cell 51, 559-72. 15 . Kawamata, S. et al. (1998) J Biol Chem 273, 5808-14. |
Western blot analysis of extracts from SK-BR-3 cells, mock transfected (-) or transfected with siRNA against human TRAF4, using TRAF4 (D1N3A) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human TRAF4 (hTRAF4-Myc/DDK; +), using TRAF4 (D1N3A) Rabbit mAb (upper) or Myc-Tag (71D10) Rabbit mAb #2278 (lower). | |
Western blot analysis of extracts from various cell lines using TRAF4 (D1N3A) Rabbit mAb. |
