| 货号 | 53385S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | H Mk |
| 来源宿主 | Rabbit IgG |
| 应用 | W IP |
| 目标/特异性 | WDR59 (D4Z7A) rabbit mAb recognizes endogenous levels of total WDR59 protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The mTORC1 kinase complex plays a critical role in cell growth regulation (1, 2). mTORC1 activity is modulated by cellular environmental factors (e.g., energy levels, growth factors, amino acids) (3, 4), which are mediated through a protein complex known as GATOR (GAP Activity Towards Rags) (5). GATOR is comprised of two protein subcomplexes (GATOR1 and GATOR2), that function in opposing fashion to regulate mTORC1 activity (5). WDR59 was identified as a component of the GATOR2 subcomplex (also containing Mios, WDR24, Seh1L and Sec13), that functions to positively regulate mTORC1 activity (5). Conversely, the GATOR1 subcomplex (containing DEPDC5, Nprl2 and Nprl3), negatively regulates mTORC1 activity, through activation of RagA and RagB GTPases (5). |
| 运输条件 | 0.75 |
| 存放说明 | -20C |
| 计算分子量 | 110 |
| 参考文献 | 1 . Sancak, Y. et al. (2008) Science 320, 1496-501. 2 . Kim, E. et al. (2008) Nat Cell Biol 10, 935-45. 3 . Hay, N. and Sonenberg, N. (2004) Genes Dev 18, 1926-45. 4 . Wullschleger, S. et al. (2006) Cell 124, 471-84. 5 . Bar-Peled, L. et al. (2013) Science 340, 1100-6. |
Western blot analysis of extracts from various cell lines using WDR59 (D4Z7A) Rabbit mAb. | |
Immunoprecipitation of WDR59 from MCF7 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is WDR59 (D4Z7A) Rabbit mAb. Western blot analysis was performed using WDR59 (D4Z7A) Rabbit mAb. |
