| 货号 | 92529S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Mouse |
| 应用 | WB,CHIP |
| 目标/特异性 | TET2 (D9K3E) Rabbit mAb (Mouse Specific) recognizes endogenous levels of total mouse TET2 protein. |
| 使用方法 | Western Blotting (1:1000) Chromatin IP (1:50) Chromatin IP-seq (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Methylation of DNA at cytosine residues is a heritable, epigenetic modification that is critical for proper regulation of gene expression, genomic imprinting, and mammalian development (1,2). 5-methylcytosine is a repressive epigenetic mark established de novo by two enzymes, DNMT3a and DNMT3b, and is maintained by DNMT1 (3, 4). 5-methylcytosine was originally thought to be passively depleted during DNA replication. However, subsequent studies have shown that Ten-Eleven Translocation (TET) proteins TET1, TET2, and TET3 can catalyze the oxidation of methylated cytosine to 5-hydroxymethylcytosine (5-hmC) (5). Additionally, TET proteins can further oxidize 5-hmC to form 5-formylcytosine (5-fC) and 5-carboxylcytosine (5-caC), both of which are excised by thymine-DNA glycosylase (TDG), effectively linking cytosine oxidation to the base excision repair pathway and supporting active cytosine demethylation (6,7). TET2 is the most frequently mutated gene in myeloid dysplastic syndrome (MDS), a dysplasia of myeloid, megakaryocytic, and/or erythroid cell lineages, of which 30% progress to acute myeloid leukemia (AML) (8, 9). It is also mutated in diffuse large B-cell lymphoma (10). TET2 protein expression is often reduced in solid tumors such as prostate cancer, melanoma, and oral squamous cell carcinoma (11-13). |
| 存放说明 | -20C |
| 计算分子量 | 280 |
| 参考文献 | 1 . Hermann, A. et al. (2004) Cell Mol Life Sci 61, 2571-87. 2 . Turek-Plewa, J. and Jagodziński, P.P. (2005) Cell Mol Biol Lett 10, 631-47. 3 . Okano, M. et al. (1999) Cell 99, 247-57. 4 . Li, E. et al. (1992) Cell 69, 915-26. 5 . Tahiliani, M. et al. (2009) Science 324, 930-5. 6 . He, Y.F. et al. (2011) Science 333, 1303-7. 7 . Ito, S. et al. (2011) Science 333, 1300-3. 8 . Langemeijer, S.M. et al. (2009) Nat Genet 41, 838-42. 9 . Yamazaki, J. et al. (2012) Epigenetics 7, 201-7. 10 . Asmar, F. et al. (2013) Haematologica 98, 1912-20. 11 . Nickerson, M.L. et al. (2013) Hum Mutat 34, 1231-41. 12 . Lian, C.G. et al. (2012) Cell 150, 1135-46. 13 . Jäwert, F. et al. (2013) Anticancer Res 33, 4325-8. |
Western blot analysis of extracts from various cell lines using TET2 (D9K3E) Rabbit mAb (Mouse Specific) (upper) and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). As expected, mES and F9 cells are positive for TET2 expression, while NIH/3T3 and C2C12 cells are negative. | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from mES cells and TET2 (D9K3E) Rabbit mAb (Mouse Specific) using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA libraries were prepared from 5 ng enriched ChIP DNA using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®, and sequenced on the Illumina NextSeq. The figure shows binding across the human Zmym6 gene. For additional ChIP-seq tracks, please download the product data sheet. | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from mES cells and either TET2 (D9K3E) Rabbit mAb (Mouse Specific) or Normal Rabbit IgG #2729 using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. The enriched DNA was quantified by real-time PCR using SimpleChIP® Mouse mTULP4 Promoter Primers #89052, mouse ASAH3L promoter primers, and SimpleChIP® Mouse MYT-1 Promoter Primers #8985. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. |
