| 货号 | 19436S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit IgG |
| 应用 | WB |
| 目标/特异性 | HELQ (D4K2O) Rabbit mAb recognizes endogenous levels of total HELQ protein. In some cell lines, this antibody cross-reacts with a 200 kDa band of unknown identity by western blot. |
| 使用方法 | W |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | DNA interstrand crosslinks (ICLs) are toxic DNA lesions caused by environmental agents as well as some chemotherapeutic drugs. These lesions are repaired via multiple DNA repair pathways. HELQ (also known as HEL308) is a 3’-5’ DNA helicase that colocalizes with stalled DNA replication forks in response to DNA damage, and contributes to the repair of ICL lesions through ATR signaling (1,2). HELQ interacts with various DNA repair proteins, including FANCD2, RAD51, RAD51 paralogs, and ATR (1-3). HELQ-deficient mice exhibit reduced fertility as well as interstrand crosslink (ICL) repair defects, and are prone to tumors (3). |
| 存放说明 | -20C |
| 计算分子量 | 130 |
| 参考文献 | 1 . Tafel, A.A. et al. (2011) J Biol Chem 286, 15832-40. 2 . Takata, K. et al. (2013) Nat Commun 4, 2338. 3 . Adelman, C.A. et al. (2013) Nature 502, 381-4. |
Western blot analysis of extracts from various cell lines using HELQ (D4K2O) Rabbit mAb. | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing DDK-tagged full-length human HELQ (hHELQ-DDK; +), using HELQ (D4K2O) Rabbit mAb (upper) or DYKDDDDK Tag (D6W5B) Rabbit mAb #14793 (lower). | |
Western blot analysis of extracts from wild type or HELQ-knockout U-2 OS cells using HELQ (D4K2O) Rabbit mAb (upper) or α-Actinin (D6F6) XP® Rabbit mAb #64873 (lower). |
