| 货号 | 14334S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P |
| 使用方法 | WB(1:1000) IP (1:100) IHC-P (1:50) |
| 供应商 | CST |
| 背景 | The inhibitor of apoptosis protein (IAP) family consists of an evolutionarily conserved group of apoptosis inhibitors containing a conserved 70 amino acid BIR (baculovirus inhibitor repeat) domain (1,2). Human members of this family include c-IAP1, c-IAP2, XIAP, survivin, livin, and NAIP. Overexpression of IAP family members, particularly survivin and livin, in cancer cell lines and primary tumors suggests an important role for these proteins in cancer progression (3-5). In general, the IAP proteins function through direct interactions to inhibit the activity of several caspases, including caspase-3, caspase-7, and caspase-9 (5,6). In addition, binding of IAP family members to the mitochondrial protein Smac blocks their interaction with caspase-9, thereby allowing the processing and activation of the caspase (2). |
| 存放说明 | -20C |
| 计算分子量 | 53 |
Immunohistochemical analysis of paraffin-embedded HT-29 cells, untreated (left) or mithramycin A-treated (right), using XIAP (D2Z8W) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using XIAP (D2Z8W) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using XIAP (D2Z8W) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). | |
Western blot analysis of extracts from various cell lines using XIAP (D2Z8W) Rabbit mAb. | |
Western blot analysis of extracts from HT-29 cells, untreated (-) or treated with mithramycin A (200 nM, overnight; +) using XIAP (D2Z8W) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Immunoprecipitation of XIAP from MCF7 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or XIAP (D2Z8W) Rabbit mAb (lane 3). Lane 1 represents 10% input. Western blot was performed using XIAP (D2Z8W) Rabbit mAb. The conformation specific secondary antibody Mouse Anti-Rabbit IgG (Conformation Specific) (L27A9) mAb #3678 was using to avoid cross reactivity with the IgG heavy chain. |
