| 货号 | 43130S |
| 反应种属 | Human |
| 来源宿主 | Rabbit IgG |
| 应用 | W/IP/IF-IC |
| 使用方法 | WB(1:1000) IP (1:100) IF-IC (1:1000) |
| 供应商 | CST |
| 背景 | Angiomotin (AMOT) is a widely expressed cell junction protein initially identified through its ability to bind angiostatin. Alternative splicing results in two isoforms of angiomotin, the full-length p130 and the amino-terminally truncated p80. These isoforms, along with angiomotin-like 1 (AmotL1) and angiomotin-like 2 (AmotL2) comprise the Motin protein family. Angiomotin is important in endothelial cell polarity, migration and blood vessel formation during development, as well as in signaling through small GTPases and the Hippo/YAP pathway (reviewed in 1). |
| 存放说明 | -20C |
| 计算分子量 | 80, 140 |
Confocal immunofluorescent analysis of 293T (left) or MCF7 cells (right), using Angiomotin (D2O4H) Rabbit mAb (green). Actin filaments were labeled with DyLightTM 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Immunoprecipitation of Angiomotin from 293T cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Angiomotin (D2O4H) Rabbit mAb. Western blot analysis was performed using Angiomotin (D2O4H) Rabbit mAb. | |
Western blot analysis of extracts from 293T and MCF7 cells, using Angiomotin (D2O4H) Rabbit mAb (upper) or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human Angiomotin p80 (hAngiomotin p80-Myc/DDK; +) protein, using Angiomotin (D2O4H) Rabbit mAb (upper) or Myc-Tag (71D10) Rabbit mAb #2278 (lower). |
