| 货号 | 13507S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Atg4B (D1G2R) Rabbit mAb recognizes endogenous levels of total Atg4B protein. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Control of autophagy was largely discovered in yeast and involves proteins encoded by a set of autophagy-related genes (Atg) (1). Formation of autophagic vesicles requires a pair of essential ubiquitin-like conjugation systems, Atg12-Atg5 and Atg8-phosphatidylethanolamine (Atg8-PE), which are widely conserved in eukaryotes (2). Numerous mammalian counterparts to yeast Atg proteins have been described, including three Atg8 proteins (GATE-16, GABARAP, and LC3) and four Atg4 homologs (Atg4A/autophagin-2, Atg4B/autophagin-1, Atg4C/autophagin-3, and Atg4D/autophagin-4) (3-5). The cysteine protease Atg4 is pivotal to autophagosome membrane generation and regulation. Atg4 primes the Atg8 homolog for lipidation by cleaving its carboxy terminus and exposing its glycine residue for E1-like enzyme Atg7. The Atg8 homolog is transferred to the E2-like enzyme Atg3 before forming the Atg8-PE conjugate. During later stages of autophagy, Atg4 can reverse this lipidation event by cleaving PE, thereby recycling the Atg8 homolog (6). |
| 存放说明 | -20C |
| 计算分子量 | 48 |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing full-length mouse Atg4B (mAtg4B; +), using Atg4B (D1G2R) Rabbit mAb. Western blot 分析293T细胞的细胞提取物，未转染（-）对照或转染了表达全长鼠Atg4B (mAtg4B; +)的载体，使用抗体是Atg4B (D1G2R) Rabbit mAb 兔单抗。 | |
Immunoprecipitation of Atg4B from RD cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype control #3900 (lane 2) or Atg4B (D1G2R) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Atg4B (D1G2R) Rabbit mAb. | |
对RD细胞提取物中的Atg4进行免疫共沉淀，使用抗体是Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (泳道2) 或 Atg4B (D1G2R) Rabbit mAb 兔单抗 (泳道3)。泳道1为10%的上样。Western blot 分析使用抗体是Atg4B (D1G2R) Rabbit mAb 兔单抗。 |
