| 货号 | 80775S |
| 反应种属 | Human |
| 来源宿主 | Rabbit IgG |
| 应用 | W/IP/ChIP |
| 使用方法 | WB(1:1000) IP (1:200) ChIP (1:50) |
| 供应商 | CST |
| 背景 | The transcription regulator BTB and CNC homolog 2 (BACH2) is a bZIP domain-containing transcriptional repressor that dimerizes with MafK and binds Maf recognition elements (MAREs) to regulate transcription (1,2). BACH2 is part of a network of transcription factors that controls the transition of activated B cells into either antibody-producing plasma cells or memory B cells (3-5). Plasma cell differentiation requires the transcription factor Blimp1 (6). BACH2 suppresses expression of Blimp1 in activated B cells, which delays plasma cell differentiation and allows time for class switch recombination and somatic hypermutation (3-5). Genome-wide association studies have linked the genetic locus containing BACH2 to several immune-related disorders including type 1 diabetes, celiac disease, Crohn’s disease, and the skin condition known as vitiligo (7-10). |
| 存放说明 | -20C |
| 计算分子量 | 130 |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 RL-7 cells and either 10 μl of BACH2 (D3T3G) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human PRDM1 promoter primers, SimpleChIP® Human HMOX1 Promoter Primers #53538, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. | |
Western blot analysis of extracts from various cell lines using BACH2 (D3T3G) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Immunoprecipitation of BACH2 from Daudi cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is BACH2 (D3T3G) Rabbit mAb. Western blot analysis was performed using BACH2 (D3T3G) Rabbit mAb. |
