| 货号 | 14020S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/ChIP |
| 使用方法 | WB(1:1000) IP (1:50) ChIP (1:50) |
| 供应商 | CST |
| 背景 | Circadian rhythms govern many key physiological processes that fluctuate with a period of approximately 24 hours. These processes include the sleep-wake cycle, glucose, lipid and drug metabolism, heart rate, hormone secretion, renal blood flow, and body temperature, as well as basic cellular processes such as DNA repair and the timing of the cell division cycle (1,2). The mammalian circadian system consists of many individual tissue-specific clocks (peripheral clocks) that are controlled by a master circadian pacemaker residing in the suprachiasmatic nuclei (SCN) of the brain (1,2). The periodic circadian rhythm is prominently manifested by the light-dark cycle, which is sensed by the visual system and processed by the SCN. The SCN processes the light-dark information and synchronizes peripheral clocks through neural and humoral output signals (1,2). |
| 存放说明 | -20C |
| 计算分子量 | 78 |
Western blot analysis of extracts from various cell lines and tissues using BMAL1 (D2L7G) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Immunoprecipitation of BMAL1 from SH-SY5Y cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or BMAL1 (D2L7G) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using BMAL1 (D2L7G) Rabbit mAb. | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc-tagged full-length human BMAL1 protein (hBMAL1-Myc; +), using BMAL1 (D2L7G) Rabbit mAb. | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 3T3 cells treated with dexamathasone (1 μM) for 2 hours, and either 10 μl of BMAL1 (D2L7G) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using mouse DBP intron 2 primers, SimpleChIP® Mouse PER1 Intron 1 Primers #13902, and SimpleChIP® Mouse RPL30 Intron 2 Primers #7015. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. |
