| 货号 | 12137S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-F/ChIP |
| 目标/特异性 | Brn2/POU3F2 (D2C1L) Rabbit mAb recognizes endogenous levels of total Brn2/POU3F2 protein. |
| 使用方法 | WB(1:1000) IP (1:100) IF-F (1:3200) ChIP (1:50) |
| 供应商 | CST |
| 背景 | Brn2/POU3F2 is a POU domain-containing transcription factor involved in neuronal differentiation and activation of the corticotrophin-releasing hormone gene (1,2). In mice, disruption of the Brn2 gene results in loss of specific neuronal lineages in the hypothalamus (3). In addition to its role in mammalian neurogenesis, Brn2 has also been implicated in melanoma tumorigenesis and has been shown in the literature to be overexpressed in human melanoma cells compared to normal melanocytes (4,5). Recent studies also identify Brn2 as a transcription factor playing an important role in keratinocyte differentiation (6). Recent reports demonstrate that overexpression of three transcription factors (Brn2, Ascl1, and Myt1L) can directly convert human fibroblasts into functional neurons under precisely defined conditions (7,8). |
| 存放说明 | -20C |
| 计算分子量 | 55 |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 A-375 cells and either 10 μl of Brn2/POU3F2 (D2C1L) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human MITF Promoter Primers #12377 and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. | |
染色质免疫共沉淀分析来源于4 x 106 A-375细胞的交联染色质。使用的抗体是10 μl的Brn2/POU3F2 (D2C1L) Rabbit mAb 兔单抗或 2 μl的Normal Rabbit IgG #2729，使用SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003。QPCR分析富集的DNA，使用试剂盒为SimpleChIP® Human MITF Promoter Primers #12377和SimpleChIP® Human α Satellite Repeat Primers #4486。在每个样品中免疫沉淀的DNA量被定义为与基因组内参对照(相当于1)总量相关的信号。 |
