货号 | 16984S |
反应种属 | Human |
来源宿主 | Rabbit IgG |
应用 | W/IP/IHC-P/IF-IC |
使用方法 | WB(1:1000) IP (1:100) IHC-P (1:500) IF-IC (1:200) |
供应商 | CST |
背景 | The coxsackie virus and adenovirus receptor (CXADR, CAR) is a highly conserved, single-transmembrane glycoprotein and the primary receptor to mediate cellular attachment and infection of coxsackie B viruses and most adenoviruses (1,2). The CAR protein contains a pair of Ig-like domains within the amino-terminal extracellular domain and a carboxyl-terminal PDZ motif (1). Research studies indicate that CAR is a tight junction protein that associates with the ZO-1 scaffold protein and promotes both cell adhesion and restriction of solute and ion movement between cells (2). Endogenous CAR is targeted to the basolateral plasma membrane by a tyrosine-based basolateral sorting signal and clathrin adaptors AP-1A and AP-1B (3). CAR binds junctional adhesion molecule L (JAML) on epithelial cells and neutrophils where it activates PI3K and downstream MAPK kinases to stimulate epithelial γδ T cell proliferation and increase production of TNFα and keratinocyte growth factor (4-6). As a result, the CAR protein plays a potentially critical role in adenoviral gene therapy, immunity, wound repair, inflammation, and cancer therapy (4-6). Additional studies demonstrate that CAR is essential in regulating squamous carcinoma cell growth (7). |
存放说明 | -20C |
计算分子量 | 45-55 |
Confocal immunofluorescent analysis of Hep G2 (left) and RD (right) cells, using CAR (D3W3G) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using CAR (D3W3G) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded Hep G2 (left) and K-562 (right) cell pellets using CAR (D3W3G) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using CAR (D3W3G) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). | |
Immunoprecipitation of CAR protein from HCT-15 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is CAR (D3W3G) Rabbit mAb. Western blot analysis was performed using CAR (D3W3G) Rabbit mAb. | |
Western blot analysis of extracts from HCT-15, LoVo, and HCC1419 cell lines using CAR (D3W3G) Rabbit mAb. |